Safety Monitoring

Determination of 37 dyes in hair dye products with quantitative analysis of multi-components by single marker

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  • Beijing Institute for Drug Control, Beijing 102206, China

Received date: 2022-02-28

  Online published: 2024-06-24

Abstract

Objective: To establish a method for simultaneous determination of 37 kinds of hair dyes such as p-phenylenediamine in oxidized hair dye products with quantitative analysis of multi-components by single marker (QAMS), and verify its applicability and accuracy in the quality control of hair dye products. Methods: The analytes were separated by high performance liquid chromatography (HPLC). TechMate AQUA-STⅢ(250 mm×4.6 mm,5 μm) column was used and a mixture of the ammonium acetate solution containing 4% acetonitrile and acetonitrile was used as mobile phase for gradient elution. The determination was carried out at 235 nm and 280nm by diode array detector. m-aminophenol was used as the internal standard to determine the relative correction factors (fsi) of the 36 other hair dyes. Contents of the dyes in sample were calculated by their fsi. Results: Good linearity with correlation coefficients greater than 0. 999 was obtained for all the analytes. Recoveries of the method were from 81.7% to 114.8% at three concentration levels, with the reproducibility RSD were less than 5.0% (n=6). The contents of hair dyes in 6 batches of oxidative hair dye products measured by QAMS method were not significantly different from those using the standard curve method (SCM). The relative average deviation (RAD) were less than 4%. Conclusion: The established QAMS method with simple operation, accurate results and saving reference materials, which can quantitatively analyze 37 kinds of hair dyes in oxidized hair dye products at the same time. It can provide reference for quality evaluation and risk monitoring of oxidized hair dye products.

Cite this article

DI Zheng, ZUO Xue, WANG Ya-jing, YANG Ling, WU Guo-qing, DU Yong . Determination of 37 dyes in hair dye products with quantitative analysis of multi-components by single marker[J]. Chinese Journal of Pharmaceutical Analysis, 2023 , 43(2) : 314 -323 . DOI: 10.16155/j.0254-1793.2023.02.15

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