Objective: To develop a SPE-isotope dilution GC-MS/MS method for the determination of the residues of 18 polycyclic aromatic hydrocarbons(PAHs) in root and rhizome herbs. Methods: Using isotope as internal standard, the sample was extracted by acetonitrile and purified by solid phase extraction on C18 cartridges. GC-MS/MS method was used for the assay. The chromatographic column was DB-17ms(30 m ×0.25 mm, 0.25 μm) with temperature programming and MRM detection. Results: The calibration curves for the 18 kinds of typical PAHs were linear in the range of 2-50 ng·mL-1. The average recovery rate was in the range of 63.8%-118.0%, with RSD 0.45%-19.0%(n=3). The LOQs were 0.5-1 μg·kg-1. Conclusion: This method is specific, sensitive, and can be used for residue detection of 18 typical kinds of PAHs in root and rhizome herbs.
LIU Li-na, JIN Hong-yu, ZAN Ke, MA Shuang-cheng
. Determination of 18 polycyclic aromatic hydrocarbons in root and rhizome herbs by SPE-isotope dilution GC-MS/MS[J]. Chinese Journal of Pharmaceutical Analysis, 2023
, 43(3)
: 448
-456
.
DOI: 10.16155/j.0254-1793.2023.03.11
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