Objective: To establish an HPLC method and chemical pattern recognition model of poeny leaves from Henan province, providing quality evaluation of poeny leaves. Methods: Analysis was performed on an Agilent C18 column (250 mm×4.6 mm, 5 μm) with acetonitrile-0.1% formic acid for gradient elution. The flow rate was 1.0 mL·min-1, the detection wavelength was set at 270 nm, and the column temperature was 35 ℃. “Similarity Evaluation System of Fingerprint of Tradition Chinese Medicine (2012 edition)” was adopted to establish reference fingerprint and to calculate the similarities of 21 batches of poeny leaves. Combining with principal component analysis and cluster analysis, the overall quality of poeny leaves was evaluated. Results: There were 18 common characteristic peaks in the fingerprints of the 21 batches of samples, 5 peaks were identified as gallic acid, ethylgallate, paeoniflorin, ellagic acid and luteoloside. Similarities of 21 batches of samples were between 0.907-0.979. Samples were clustered into 3 types by principal component analysis which consistented with cluster analysis result. Conclusion: The method combining HPLC fingerprint with chemical pattern recognition is simple and reliable, which can quickly evaluate the quality and consistency of different resources of poeny leaves from Henan province, and is suitable for the evaluation of the overall quality evaluation of poeny leaves.
NING Er-juan, CHEN Ling, WANG Xue-fang, ZHANG Li-xian, LI Xiao, ZHANG Li-pan, WANG Fa-yun
. HPLC fingerprint combined with chemical pattern recognition for quality evaluation of poeny leaves from Henan province*[J]. Chinese Journal of Pharmaceutical Analysis, 2023
, 43(3)
: 486
-493
.
DOI: 10.16155/j.0254-1793.2023.03.16
[1] 赵兰勇. 中国牡丹栽培与鉴赏[M].北京: 金盾出版社, 2004: 1, 223
ZHAO LY. Peony Cultivation and Appreciation in China[M].Beijing: Jindun Publishing Company, 2004: 1, 223
[2] 包雅婷. 紫斑牡丹花、叶抗氧化与抗菌活性的研究[D].成都:西南民族大学, 2018
BAO YT. Antioxidant and Antibacterial Activity of Flowers and Leaves from Paeonia rockii[D].Chengdu:Southwest Minzu University, 2018
[3] 董丽梅, 赵秀娟, 刘姚, 等. 油用牡丹不同部位化学成分与开发利用研究进展[J].现代食品科技, 2021, 37(10):348
DONG LM, ZHAO XJ, LIU Y, et al. Research progress on chemical constituents and utilization of different parts of oil peony[J].Mod Food Sci Technol, 2021, 37(10):348
[4] 夏成凯, 詹云武, 胡云飞, 等. HPLC 法同时测定不同产地牡丹皮中13种化学成分的含量[J].中草药, 2019, 50(4):970
XIA CK, ZHAN YW, HU YF, et al. Simultaneous determination of thirteen components of Mortan Cortex in different regions by HPLC[J].Chin Tradit Herb Drugs, 2019, 50(4):970
[5] 王新娣, 石晓峰, 王斌利, 等. 牡丹化学成分的研究进展[J].中成药, 2018, 40(1):171
WANG XD, SHI XF, WANG BL, et al. Research progress of chemical components from Paeonia suffruticosa Andr.[J].Chin Tradit Pat Med, 2018, 40(1):171
[6] 陈箫箫, 赵宏苏, 章芳珍, 等. 安徽主产区牡丹皮药材质量差异研究[J].中南药学, 2020, 18(4):680
CHEN XX, ZHAO HS, ZHANG FZ, et al. Quality difference in Moutan Cortex from Anhui province[J].Cent South Pharm, 2020, 18(4):680
[7] 袁琴琴, 刘文营. 牡丹籽中营养和功能性成分研究进展[J].食品工业科技, 2019, (17):333
YUAN QQ, LIU WY. Research progress on the nutritional and functional components of peony (Paeonia suffruticosa Andr.) seeds[J].Sci Technol Food Ind, 2019, (17):333
[8] 刘玉军, 索俊玲, 孙志强, 等. 牡丹籽活性成分及综合开发利用研究进展[J].食品与药品, 2020, 22 (4):321
LIU YJ, SUO JL, SUN ZQ, et al. Progress in active components and comprehensive utilization of peony seeds[J].Food Drug, 2020, 22 (4):321
[9] 张岩松, 刘向慧, 牛江秀, 等. 护肤功效原料牡丹花和牡丹叶提取物的提取工艺和性能研究[J].精细与专用化学品, 2020, 28(12):27
ZHANG YS, LIU XH, NIU JX, et al. Study on optimum extraction process of peony flower extracts and leaf extracts and its application as cosmetic raw materials for skin care[J].Fin Spec Chem, 2020, 28(12), 27
[10] 马晓, 陈刚, 梁盼. “凤丹”牡丹叶轴多糖超声辅助提取工艺优化及抗氧化性研究[J].食品研究与开发, 2019, 40(12):126
MA X, CHEN G, LIANG P. Optimization of polysaccharide extraction from Paeonia suffruticosa ‘Fengdan’ Rachises by ultrasonic-assisted method and its antioxidant ability[J].Food Res Devel, 2019, 40(12):126
[11] 高志情, 张岩松, 任利鹏, 等. 牡丹叶的研究进展及应用[J].云南化工, 2021, 48(11):18
GAO ZQ, ZHANG YS, REN LP, et al. Research progress and application of peony leaves[J].Yunnan Chem Technol, 2021, 48(11):18
[12] 杜敬泉, 郭晓玲, 魏永利, 等. 基于网络药理学浅析牡丹叶治疗宫颈癌的分子机制[J].国际肿瘤学杂志, 2018, 45(9):519
DU JQ, GUO XL, WEI YL, et al. Network pharmacology based study on mechanism of peony leaf in the treatment of cervical cancer[J].J Int Oncol, 2018, 45(9):519
[13] 郭晓玲, 李爱华, 李克明, 等. 牡丹叶治疗原发性痛经机制研究[J].河南中医, 2018, 38(7):1109
GUO XL, LI AH, LI KM, et al. Study on mechanism of peony leaf in the treatment of primary dysmenorrheal[J].Henan Tradit Chin Med, 2018, 38(7):1109
[14] 张育贵, 张淑娟, 边甜甜, 等. 芍药苷药理作用研究新进展[J].中草药, 2019, 50(15):3735
ZHANG YG, ZHANG SJ, BIAN TT, et al. New progress in pharmacological action of paeoniflorin[J].Chin Tradit Herb Drugs, 2019, 50(15):3735
[15] 山东省中药材标准[S].2012: 129
Standard for Chinese Medicinal Materials in Shandong Province[S].2012:129
