Ingredient Analysis

Ion-pairing RP-HPLC method to determine tetrahydrocurcumin*

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  • 1. School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China;
    2. Sichuan Academy of Chinese Medicine Science, Chengdu 610041, China

Received date: 2021-09-16

  Online published: 2024-06-24

Abstract

Objective: To establish an ion-pairing reverse phase HPLC determination method for detecting the concentration of tetrahydrocurcumin in active pharmaceutical ingredient(API). Methods: Analysis was carried out with an Agilent ZORBAX Extend-C18(150 mm×4.6 mm,5.0 μm)column; the mobile phase was composed of acetonitrile-0.05% amnonia and 10 mmol·L-1 dodecyl trimethyl ammonium chloride in water(38∶62), with linear gradient elution at a flow rate of 1 mL·min-1. The detection wavelength was 288 nm. The column temperature was 25 ℃. Results: The linear range of the calibration curve for tetrahydrocurcumin was 0.149-7.452 μg(r=0.999 9). The average recovery was 99.9%(RSD=0.68%).Limits of quantity and detection were 0.011 μg and 0.044 μg, respectively. Tetrahydrocurcumin and its related substances were completely separated. According to this method, the contents of three batches of API were 98.6%, 98.3% and 98.3%, respectively. Conclusion: Because of its simplicity, accuracy and specificity, this method is suitable for the determination of content of tetrahydrocurcumin API.

Cite this article

ZHU Ning, LING Hai-yan, YANG An-dong, ZHAO Jun-ning, LI Xiao-fang . Ion-pairing RP-HPLC method to determine tetrahydrocurcumin*[J]. Chinese Journal of Pharmaceutical Analysis, 2022 , 42(5) : 773 -779 . DOI: 10.16155/j.0254-1793.2022.05.04

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