Objective: To establish an UPLC-MS/MS method for simultaneous determination of nifedipine and its two degradation products(dehydro nifedipine and dehydronitroso nifedipine) added illegally in food and health food. Methods: Samples were extracted by acetonitrile aqueous solution containing 5% formic acid. The analysis was performed on C18(50 mm×2.1 mm,1.8 μm)column with gradient elution using a mobile phase of 0.1% aqueous formic acid(2 mmol·L-1 ammonium formate)and acetonitrile at 0.4 mL·min-1. Mass spectrometry acquisition was done in the positive ion mode and analytes were detected in the MRM mode. Results: Three chemical components could be separately well and have a good linear relationship in 2-100 μg·L-1 range. The average recovery rates of low,medium and high concentration were 90.9%-103.0% with the RSD of 1.2%-6.1%. The detection limits and quantitation limits of each component in various matrices were lower than 0.05 mg·kg-1 and 0.1 mg·kg-1, respectively. Conclusion: The developed method verified by the methodology is precise, sensitive, accurate, which therefore can be applied for the analysis of illegally added in food health food.
NING Xiao, JING Shao-ming, DONG Zhe, ZHAO Yun-feng, CAO Jin
. Determination of nifedipine illegally adulterated in food and health foods by UPLC-MS/MS*[J]. Chinese Journal of Pharmaceutical Analysis, 2022
, 42(5)
: 821
-830
.
DOI: 10.16155/j.0254-1793.2022.05.11
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