Quality Control

Quality evaluation of Corydalis Rhizoma processed by different methods based on combinative methods of HPLC fingerprint, quantitative analysis of multi-components and chemometric analysis*

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  • Nantong Health College of Jiangsu Province, Nantong 226010, China

Received date: 2021-06-03

  Online published: 2024-06-24

Abstract

Objective: To establish the HPLC fingerprint of Corydalis Rhizoma and vinegar Corydalis Rhizoma, and to provide reference for comprehensive evaluation of the quality by chemometric method. Methods: The samples were determined by HPLC. An Agilent XDB C18 column (250 mm×4.6 mm,5 μm) was used with acetonitrile -0.1% phosphoric acid solution (gradient elution) as the mobile phase, and the detection wavelength was 280 nm. The HPLC fingerprints of Corydalis Rhizoma and vinegar Corydalis Rhizoma were established by using “The similarity evaluation system of traditional Chinese medicine chromatographic fingerprint” (2012 A version), and determination method of six compounds was validated. The fingerprints were analyzed by PLS-DA. Results: There were 17 and 20 common peaks in the fingerprints of Corydalis Rhizoma and vinegar Corydalis Rhizoma, respectively. The similarities were 0.934-0.985 and 0.954-0.987, respectively. Six compounds were identified as protopine, palmatine hydrochloride, berberinehydrochloride, dehydrocorydaline, tetrahydropalmatine and corydaline by comparison with the reference substances. The linear relationship of the six components in the range of their respective mass concentration was good (r2>0.999), the average recovery of addition were 100.2%-104.3% and the RSDs were 0.94%-1.8%. 30 samples were classified into two groups and 10 components were selected as the main differential markers by PLS-DA. Conclusion: The fingerprints of Corydalis Rhizoma and vinegar Corydalis Rhizoma and the content determination method of six compounds are successfully established, which can provide reference for processing and quality control of Corydalis Rhizoma.

Cite this article

XU Rong, SHI Tian-hui . Quality evaluation of Corydalis Rhizoma processed by different methods based on combinative methods of HPLC fingerprint, quantitative analysis of multi-components and chemometric analysis*[J]. Chinese Journal of Pharmaceutical Analysis, 2022 , 42(5) : 856 -865 . DOI: 10.16155/j.0254-1793.2022.05.15

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