Quality Control

Establishment of fingerprint and identification of chemical pattern of Bufonis Coriumand processed products*

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  • Nantong Food and Drug Control Center, Nantong 226006, China

Received date: 2021-05-18

  Online published: 2024-06-24

Abstract

Objective: To control the quality of Bufonis Corium with chemical pattern, UPLC fingerprints of raw materials and processed products of Bufonis Corium were establish and the contents of the index components of Bufonis Corium, such as gamafotalin, arenobufagin, bufotalidin, bufotaline, cinobufotalin, bufalin, cinobufagin, resibufogenin and 3-arenobufagyl-subericacid were determined. Methods: The methanol extract of dry ecdysis was analyzed by Waters Acquity UPLC BEH chromatographic column (100 mm×2.1 mm, 1.7 μm) with a mobile phase of acetonitrile -0.5% acetic acid aqueous solution in gradient elution.The flow rate was 0.2 mL·min-1, the column temperature was 30 ℃ and the detection was set at 296 nm. Results: The total contents of 12 batches of raw products and 12 batches of processed products were 3.18-4.69 mg·g-1 and 0.91-2.77 mg·g-1, there were 9 common peaks in the fingerprints, and the similarity were 0.963-0.991 and 0.692-0.988, respectively. The principal component analysis and cluster analysis could identify the raw and processed products of Bufonis Corium. Conclusion: The method is stable and reliable, and can provide scientific basis for quality control, processing technology and further development of Bufonis Corium.

Cite this article

ZHOU Mi, XU Dan-yang, SUN Cheng-jing, QIAN Jie . Establishment of fingerprint and identification of chemical pattern of Bufonis Coriumand processed products*[J]. Chinese Journal of Pharmaceutical Analysis, 2022 , 42(5) : 875 -883 . DOI: 10.16155/j.0254-1793.2022.05.17

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