Objective: To establish a method for determining the content of 5 components in extracts of different polar parts of Angelica sinensis (div.) Diels and to investigate the intervention effect of extract of Angelica sinensis (div.) Diels on mice with blood stasis syndrome. Methods: HPLC method was used to determine the contents of 5 components in the extracts of different polar parts of Angelica sinensis (div.) Diels. KM mice were randomly divided into blank group, model group, positive control group, Angelica sinensis (div.) Diels petroleum ether group, n-butanol group, water position group and ethyl acetate group. On the first day of the experiment, the mice in the blank group and the model group were given the same amount of pure water. Results: The methodological inspections met the requirements. The average sample recovery rates of chlorogenic acid, ferulic acid, ligustalide Ⅰ, ligustilide, and angelica lactone were 99.3%, 101.0%, 98.5%, 97.2% and 99.1%, respectively with RSD values less than 3.0%. The TXB2 content decreased in Angelica sinensis (div.) Diels experimental group, and 6-keto-PGF1α content increased significantly (P<0.05). Compared with the control group, the serum t-PA content decreased, and the serum PAI-1 and cAMP content increased (P<0.05). Compared with the model group, the serum t-PA content increased, and the serum PAI-1 and cAMP content decreased (P<0.05). Compared with the model group, RBC, HGB and HCT in peripheral blood of Angelica sinensis (div.) Diels extract were significantly increased in each group and positive control group (P>0.05). Compared with model group, IL-2, IL-3, IL-6 and IL-6 in serum of Angelica sinensis (div.) Diels and positive control group were significantly increased (P>0.05), and the content of EPO was significantly increased (P<0.05). Compared with the model group, the serum levels of G-CSF, M-CSF and VCAM-1 in Angelica sinensis (div.) Diels group were significantly increased (P<0.05). Compared with the blank group and the positive control group, the spleen index of the model group was significantly increased (P<0.05), and the thymus index of the model group was significantly decreased compared with the control group and the empty white group and the positive control group (P<0.05). Conclusion: The content determination method established in this study is specific, repeatable and accurat, and can be used for the content determination of chlorogenic acid, ferulic acid, ligustalide Ⅰ, ligustilide, and angelica lactone. Angelica sinensis (div.) Diels can significantly increase the number of RBC and PLT, HGB content and HCT ratio in peripheral blood of mice. Angelica sinensis (div.) Diels can improve the expression levels of IL-3, EPO, G-CSF, M-CSF and VCMA-1, which are related to hematopoietic function in varying degrees, and has the effect of enriching blood. Angelica sinensis (div.) Diels can increase the content of 6-keto-PGF1α in serum and reduce the content of TXB2 in serum of mice, and can also improve the blood circulation Angelica sinensis (div.) Diels can maintain the dynamic balance of TXB2/6-keto-PGF1α in the blood of mice, regulate the balance of t-PA/PAI-1 in the serum, and correct the imbalance in the body of mice. Angelica sinensis (div.) Diels has the effect of nourishing blood.
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