Objective: To establish a method for the Libonotis radix mixed in Saposhnikoviae radix pieces and to test the adulteration of Saposhnikoviaeradix in the market as a supplementary inspection method. Methods: Firstly, TLC was used to analyze Saposhnikoviae radix pieces. If the characteristic spots of the characteristic components [3’(S), 4’(R)-didangryloxy-3’, 4’-dihydrozanthoxylide] of Libonotis radix was found, the quantitative analysis was carried out by HPLC. The HPLC assay was performed on a Agilent Extent C18 column (250 mm×4.6 mm, 5 μm) with acetonitrile-water(60∶40)as mobil phase by isocratic elution at a flow of 1.0 mL·min-1. The detection wavelenghth was set at 324 nm.If the value exceeds the limitation, then the Libonotis radix mixed in Saposhnikoviae radix pieces were determine. If necessary the HPLC-MS method can be used to check the 3’(S), 4’(R)-didangryloxy-3’, 4’-dihydrozanthoxylide. Results: The result showed that the characteristic components of Libonotis radix (3’(S), 4’(R)-didangryloxy-3’, 4’-dihydrozanthoxylide) could be detected and exceeds the prescribed limit in 17 of totally 236 batches of random-checked Saposhnikoviae radix, accounting for 7.2%, which was adulterated. Conclusion: TLC and HPLC methods have good specificity, repeatability, and durability and can detect the Saposhnikoviae radix adulteration quickly and effectively, and provides guarantee for the quality of Saposhnikoviae radix and its decoction pieces.
DU Juan, LI Wei, CUI Yuhong, ZHANG Lu, LIAN Yunlan, XUE Feifei, ZHANG Feng, LI Minsheng
. Experimental research on the detection methods of Libonotis radix adulteration in Saposhnikoviae radix*[J]. Chinese Journal of Pharmaceutical Analysis, 2022
, 42(6)
: 1054
-1064
.
DOI: 10.16155/j.0254-1793.2022.06.18
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