Objective: To establish a method to determine hydroquinone and phenol in whitening cosmetics by switching column two-dimensional HPLC. Methods: Two C18 columns(250 mm×4.6 mm, 5 μm)and two C16 trapping columns (20 mm×4.0 mm, 5 μm)were used with the mobile phase of 10 mmol·L-1 monopotassium phosphate solution(adjusted to pH 3.5 with phosphoric acid)-methanol in a gradient elution program at a flow rate of 1 mL·min-1. The column temperature was set at 35 ℃, the determine wavelength was 280 nm, and injection volume was 2 μL. Results: Hydroquinone and phenol showed good linear relationship in the ranges of 5.570-111.5 μg·mL-1 (r=0.999 7)and 5.410-108.1 μg·mL-1(r=0.999 6). The recoveries were 91.9%-92.6% and 94.4%-94.7% with RSDs of 3.9%-4.1% and 0.9%-2.1%, respectively. Forty batches of commercially available whitening cosmetics(including emulsion, cream, astringent, etc) were analyzed by single measurement without matrix interference. Conclusion: The method has good specificity for the determination of hydroquinone and phenol in whitening cosmetics, which can determine hydroquinone and phenol in whitening cosmetics with complex matrix rapidly and accurately.
WANG Cheng, WANG Jia-qing, ZHANG Li-wen
. Determination of hydroquinone and phenol in whitening cosmetics by column switching two-dimensional HPLC*[J]. Chinese Journal of Pharmaceutical Analysis, 2022
, 42(6)
: 1065
-1071
.
DOI: 10.16155/j.0254-1793.2022.06.19
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