Objective: To establish a quantitative analysis of multi-components by single marker method for the determination of 8 isoflavones (tectoridin, iristectorin A, iristectorin B, tectorigenin, iridin, iristectorigenin B, iristectorigenin A and irigenin) in Iris tectorum Maxim. Methods: The Agilent ZORBAX SB-C18 column (250 mm×4.6 mm, 5 μm) was used with acetonitrile as mobile phase A, water (containing 0.55% methyl-β-cyclodextrin and 0.1% phosphoric acid) as mobile phase B, and 0.1% phosphoric acid as mobile phase C. Gradient elution was performed at a flow rate of 1.0 mL·min-1, a detection wavelength of 266 nm, and a column temperature of 35 ℃. Using tectorigenin as the reference substance, relative correction factors of other ingredients were calculated, and the contents were determined. At the same time, the standard curve method was used to determine the contents of 8 components, and the differences between the two methods were compared to verify the accuracy and feasibility. Results: The linear ranges of tectoridin, iristectorin A, iristectorin B, tectorigenin, iridin, iristectorigenin B, iristectorigenin A and irigenin were 25.00-800.00, 2.50-80.00, 3.06-100.00, 1.53-50.00, 6.12-200.00, 1.53-50.00, 1.53-50.00 and 1.53-50.00 μg·mL-1, respectively. The relative correction factors of tectoridin, iristectorin A, iristectorin B, iridin, iristectorigenin B, iristectorigenin A and irigenin were 1.643, 1.445, 2.036, 1.334, 1.241, 1.548 and 1.268, respectively. The repeatability was good under different experimental conditions. Conclusion: Using tectorigenin as an the internal reference, quantitative analysis of multi-components with single marker method for 7 isoflavones were established. The method is simple, accurate and reliable, and can be used for quality evaluation and quantitative analysis of I. tectorum.
ZHOU Hongxu, ZHANG Yi, YANG Xinyong, ZENG Jun, MA Shuangcheng, MENG Dali
. Simultaneous determination of 8 isoflavones in Iris tectorum Maxim by quantitative analysis of multi-components with single marker*[J]. Chinese Journal of Pharmaceutical Analysis, 2022
, 42(6)
: 1072
-1080
.
DOI: 10.16155/j.0254-1793.2022.06.20
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