Objective: To establish a quantitative analysis of multi-components by single-marker(QAMS)method for the simultaneous content determination of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, isoforsythiaside A, quercitroside, 4,5-di-O-caffeoylquinic acid and glycyrrhizic acid in Lianhua Qingwen capsules. Methods: Analysis was performed on a Waters Acquity UPLC H-Class with PDA detector and an ACQUITY UPLC® HSS T3 column(100 mm×2.1 mm, 1.8 μm). The mobile phase consisted of methanol-acetonitrile-0.1% phosphoric acid aqueous in gradient mode. The flow rate was 0.3 mL·min-1(0-8 min) and 0.4 mL·min-1(9-60 min), the column temperature was maintained at 35 ℃ and the detection wavelengths were 205 nm(quercitroside and glycyrrhizic acid) and 327 nm(neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, isoforsythiaside A and 4,5-di-O-caffeoylquinic acid). Results: neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, isoforsythiaside A, quercitroside, 4,5-di-O-caffeoylquinic acid and glycyrrhizic acid showed good linear relationship within the ranges of 1.54-61.72, 3.88-155.16, 1.68-67.32, 4.78-191.04, 1.13-45.32, 1.49-59.52 and 2.10-84.10 μg·mL-1, respectively (r≥0.999 5). The average recoveries of the seven compounds were within 98.3%-101.2%. Results of precision, stability and reproducibility test were satisfactory. The contents of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, isoforsythiaside A, quercitroside, 4,5-di-O-caffeoylquinic acid and glycyrrhizic acid in twelve batches of samples were 1.348-2.004, 1.929-2.442, 1.118-1.881, 3.512-6.689, 0.393-0.594, 0.524-0.846 and 1.735-1.945 mg·g-1 by QAMS. There was no significant difference between the QAMS and ESM. Conclusion: The established QAMS can quantify eight components in Lianhua Qingwen capsules accurately and reliably. This developed method can be used to determine multiple components in Lianhua Qingwen capsules.
[1] 中华人民共和国药典2020年版. 一部[S].2020:1014
ChP 2020. Vol Ⅰ[S].2020:1014
[2] 王智民, 高慧敏, 付雪涛, 等.“一测多评”法中药质量评价模式方法学研究[J].中国中药杂志,2006,31(23):1925
WANG ZM,GAO HM,FU XT, et al. Multi-components quantitation by onemark new method for quality evaluation of Chinese herbal medicine[J].China J Chin Mater Med, 2006, 31(23):1925
[3] 中华人民共和国药典2010年版. 一部[S].2010:76,101,416, 1182,1493
ChP 2010. Vol Ⅰ[S].2010:76,101,416, 1182,1493
[4] 王智民, 钱忠直, 张启伟, 等. 一测多评法建立的技术指南[J].中国中药杂志, 2011, 36(6):657
WANG ZM, QIAN ZZ, ZHANG QW, et al. The guidelines for establishment of QAMS[J]China J Chin Mater Med, 2011, 36(6): 657
[5] 毕丹,孙云波,宋联强,等.连花清瘟胶囊化学成分研究(Ⅰ)[J].中草药,2018,49(4):795
BI D, SUN YB, SONG LQ, et al. Study of chemical components from Lianhua Qingwen capsule(Ⅰ)[J].Chin Tradit Herb Drugs, 2018, 49(4):795
[6] 张创峰,沈硕,宋联强,等.连花清瘟胶囊的化学成分研究(Ⅱ)[J].中草药, 2018,49(14):3222
ZHANG CF, SHEN S, SONG LQ, et al. Chemical components from Lianhua Qingwen capsules(Ⅱ)[J].Chin Tradit Herb Drugs, 2018, 49(14):3222
[7] 沈硕,张创峰,魏峰,等.连花清瘟胶囊的化学成分研究(Ⅲ)[J].中草药, 2019,50(4):814
SHEN S, ZHANG CF, WEI F, et al. Chemical components from Lianhua Qingwen capsules (Ⅲ)[J].Chin Tradit Herb Drugs, 2019,50(4): 814
[8] WANG X, LIU XT, WANG JH, et al. Study on multiple fingerprint profiles control and quantitative analysis of multi-components by single marker method combined with chemometrics based on Yankening tablets[J].Spectrochim Acta A Mol Biomol Spectrosc, 2021, 253: 119554
[9] YU P, LI Q, FENG YM, et al. Extraction and analysis of six effective components in Glycyrrhiza uralensis Fisch. by deep eutectic solvents(DES) combined with quantitative analysis of multi-components by single marker(QAMS) method[J].Molecules, 2021, 26(5): 1310
[10] 李静,李耀磊,于健东,等. 一测多评法结合特征图谱的新疆紫草质量控制研究[J].药物分析杂志,2020, 40(7):1209
LI J, LI YL, YU JD, et al. Quality control of Arnebiae Radix based on characteristic chromatogram and QAMS[J].Chin J Pharm Anal, 2020, 40(7):1209
[11] 胡瑞雪,梁元昊,徐文丽,等.一测多评法在中药中的应用及研究进展[J].药物分析杂志, 2019, 39(11):1968
HU RX, LIANG YH, XU WL, et al. Application and research progress of QAMS in traditional Chinese medicine[J].Chin J Pharm Anal, 2019, 39(11):1968
[12] 张丽先,范毅,魏悦,等.一测多评法同时测定野菊花中12个成分[J].药物分析杂志,2020,40(2):218
ZHANG LX, FAN Y, WEI Y, et al. Determination of twelve components in Chrysanthemum indicum by quantitative analysis of multi-components by single marker[J].Chin J Pharm Anal, 2020, 40(2):218
[13] 骆航,李华生.基于一测多评法的长城感冒片质量控制[J].药物分析杂志,2020, 40(3):536
LUO H, LI HS. Quality control of Changcheng Ganmao tablets based on QAMS[J].Chin J Pharm Anal, 2020, 40(3):536
[14] 张文静,王晓燕,李向阳,等.一测多评法同时测定妇科调经片中7个成分的含量[J].药物分析杂志,2020,40(2):329
ZHANG WJ, WANG XY, LI XY, et al. Simultaneous determination of seven components in Fuke Tiaojing tablets by quantitative analysis of multi-components by single marker[J].Chin J Pharm Anal, 2020, 40(2):329
[15] 刘颖新,冯传平,刘利利,等.一测多评法同时测定二至丸中8个成分[J].药物分析杂志,2021,41(2):210
LIU YX, FENG CP, LIU LL, et al. Simultaneous determination of 8 components in Erzhi pills by QAMS method[J].Chin J Pharm Anal, 2021, 41(2):210
[16] 徐阳,杨怀雷,王子龙,等.一测多评法同时测定活血丸中6个成分[J].药物分析杂志,2021,41(3):551
XU Y, YANG HL, WANG ZL, et al. Simultaneous determination of six constituents in Huoxue pills by QAMS[J].Chin J Pharm Anal, 2021, 41(3):551
[17] 何兵, 田吉, 杨世艳. 一种新的校正方式单点校正在桑叶一测多评中的应用[J].药物分析杂志, 2021, 41(7): 1133
HE B, TIAN J, YANG SY. Application of a new single-point correction method in QAMS of Mori Folium[J].Chin J Pharm Anal,2021,41(7): 1133
[18] 涂栩秀, 唐琼, 赵映娟, 等. 同时采用一测多评法和外标法测定灯盏细辛注射液中2类物质的含量[J].药物分析杂志, 2021, 41(5): 798
TU XX, TANG Q, ZHAO YJ, et al. Simultaneous determination two types of substances in Dengzhan Xixin injection by quantitative analysis of multie-components with single marker and the external standard method[J].Chin J Pharm Anal,2021,41(5): 798
[19] 陈盛君, 王协和, 陈可冀, 等. 中药复方清达颗粒标准汤剂的研究[J].药物分析杂志, 2021, 41(5): 875
CHEN SJ, WANG XH, CHEN KY, et al. Study on standard decoction of traditional Chinese medicine Qingda granules[J].Chin J Pharm Anal,2021,41(5): 875
[20] 郑云枫,李洋, 段伟萍, 等. 一测多评法测定黄芪中异黄酮及苷类成分[J].中草药, 2021, 52(10): 3104
ZHENG YF, LI Y, DUAN WP, et al. Determination of isoflavonoids and glycosides in Astragali Radix by QAMS method[J].Chin Tradit Herb Drugs, 2021,52(10): 3104
[21] 丁玉莲, 林李雁, 陈丹青, 等. 一测多评法结合双波长法分析不同产地、栽培和加工铁皮石斛黄酮类成分的含量[J].中国中药杂志, 2021, 46(14): 3605
DING YL, LIN LY, CHEN DQ, et al. Content determination of six flavonoids in Dendrobium officinale stems from different producing areas,cultivation and processing methods by QAMS combined with dual-wavelength method[J].China J Chin Mater Med,2021, 46(14): 3605
