Objective: To compare the blood pharmacokinetic behavior and urinary and fecal excretion of arsenic in normal and pseudo-sterile rats after oral administration of Realgar to clarify the influence of gut microbiota on the behavior of arsenic from Realgar in vivo. Methods: Rats were divided into normal and pseudo-sterile groups, and the pseudo-sterile rat model was established by oral gavaging of antibiotic mixtures of neomycin sulfate, streptomycin sulfate, and bacitracin.The blood, urine and feces were collected at different time points or periods after oral gavage of Realgar in normal and pseudo-sterile rats, respectively. Total arsenic content was determined by hydride generation atomic fluorescence spectroscopy(HG-AFS). The lamp current and negative pressure of arsenic were 70 mA and 250 V, respectively. The carrier and gasshield flow rate were 400 and 1 000 mL·min-1, respectively. Results: The Tmax of blood arsenic in normal and pseudo-sterile rats were 48.00 and 89.14 h, respectively. The arsenic urinary excretion in normal and pseudo-sterile rats were 76.34 and 41.24 μg, respectively. Compared with the normal rats, the Tmax of blood arsenic in pseudo-sterile rats was significantly increased, and arsenic urinary excretion was significantly reduced within 0-8 h. Conclusion: Gut microbiota accelerates the absorption of arsenic, but has no significant effect on total arsenic concentration, and promotes arsenic urinary excretion within 8 h after administration.
ZHU Hui-lin, LIN Chao, PENG Lin-xiu, WU Xiao
. Effects of rat gut microbiota on the behavior of Realgar in vivo*[J]. Chinese Journal of Pharmaceutical Analysis, 2022
, 42(7)
: 1153
-1158
.
DOI: 10.16155/j.0254-1793.2022.07.07
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