Objective: To establish a thinlayer chromatographic method which combine with solid-phase extraction for the identification of Polygoni Multiflori Radix, Dipsaci Radix, Morindae Officinalis Radix and Atractylodis Macrocephalae Rhizoma in Zishen Yutai pills. Methods: C18 solid phase extraction was used. The sample was loaded into the activated solidphase column after ultrasonic extraction, steam drying and redissolution of methanol. It was eluted with water and methanol of different concentrations successively, and the elution rate was 1 mL·min-1. The water washing was collected, evaporated the filtrate to dryness and dissolved the residue in 1 mL of methanol to produce the test solution of Morindae Officinalis Radix, a mixture of chloroform, methanol, water and glacial acetic acid (7:3:1:1) was used as the mobile phase; 60% methanol eluent was collected, evaporated the filtrate to dryness and dissolved the residue in 1ml of methanol to produce the test solution of Dipsaci Radix identification, a mixture of upper layer solution of n-butanol, acetic acid and water (4:1:5) was used as the mobile phase; 80% methanol eluent was collected, evaporated the filtrate to dryness and dissolved the residue in 1 mL of methanol to produce the test solution of Polygoni Multiflori Radix, a mixture of Cyclohexane, dichloromethane, ethyl acetate, formic acid (10:1:1:0.1) was used as the mobile phase; 100% methanol eluent was collected, evaporated the filtrate to dryness and dissolved the residue in 1 mL of methanol to produce the test solution of Atractylodis Macrocephalae Rhizoma, n-hexane, ethyl acetate, formic acid (40:10:0.5) was used as the mobile phase. Results: The fluorescent spots in the chromatogram obtained with the test solution correspond in position and colour to the spots in the chromatogram obtained with the reference drug solution and reference solution, negative samples without interference. Conclusion: Solid phase extraction can effectively enrich and analyze the low-specific components in Zishen Yutai pills. The established method has the characteristics of simple operation and good repeatability, and can be used as thin layer chromatography identification of ZishenYutai pills.
TANG Zhe, DAI Sheng-yun, WANG Wei, NING Na, LEI Ting, HE Zi-xin, QIAO Fei, ZHENG Jian, MA Shuang-cheng
. Identification of Polygoni Multiflori Radix, Dipsaci Radix, Morindae Officinalis Radix and Atractylodis Macrocephalae Rhizoma in Zishen Yutai pills by solid-phase extraction combined with thin layer chromatography[J]. Chinese Journal of Pharmaceutical Analysis, 2022
, 42(8)
: 1306
-1311
.
DOI: 10.16155/j.0254-1793.2022.08.02
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