Study on the Ethnic Medicine Column

Research on improvement of quality standard of the Guangxi ethnic herb Onychii Herba*

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  • 1. Guangxi Institute for Food and Drug Control, Nanning 530021, China;
    2. National Institutes for Food and Drug Control, Beijing 102629, China

Revised date: 2022-03-21

  Online published: 2024-06-24

Abstract

Objective: To improve the quality standard of Onychii Herba and improve its quality control level. Methods: A resource survey and field collection of Onychii Herba were carried out in Guangxi. Microscopical characteristics of 17 batches of Onychii Herba and 5 batchs of Sphenomeridis Herba were studied based on transverse section and powder. TLC was adopted to distinguish between Onychii Herba and Sphenomeridis Herba. The water content, total ash, acid-insoluble ash and ethanol soluble extractives were determined according to the method in the appendix of Chinese Pharmacopoeia. The content of cichoric acid was determined by HPLC. The CAPCELLPAK MGⅡC18 column(250 mm×4.6 mm, 5 μm) was used, the mobile phase was acetonitrile -0.3% phosphoric acid(19:81) at a flow rate of 1.0 mL·min-1, the column temperature was 25 ℃, and the detection wavelength was at 330 nm. Results: Compared with Quality Standards for Zhuang Medicine in Guangxi Zhuang Autonomous Region, microscopically characteristics of transverse section was proposed, the improved TLC showed the spots developed clearly. The linear range of cichoric acid was 2.73-273 μg·mL-1(r=1.000) and the average recovery was 102.8%(RSD=1.1%, n=6), the content of cichoric acid in 17 batches of samples ranged from 0.12% to 2.23%. Conclusion: The established method in this paper such as microscopic identification,TLC identification, inspection and content determination, improves the quality standardand provides evidence for further improvement of quality standards of Onychii Herba.

Cite this article

LIN Que-yue, DAI Sheng-yun, XIE Pei-de, TENG Ai-jun, MA Shuang-cheng, LI Li-li, ZHENG Jian . Research on improvement of quality standard of the Guangxi ethnic herb Onychii Herba*[J]. Chinese Journal of Pharmaceutical Analysis, 2022 , 42(8) : 1319 -1327 . DOI: 10.16155/j.0254-1793.2022.08.04

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