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Establishment of fingerprint of Zhengxin Jiangzhi tablets and the Q-Exactive Orbitrap HRMS analysis of its common peaks*

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  • 1. Shaanxi Academy of Traditional Chinese Medicine, Xi'an 710061, China;
    2. Xi'an C.P. Pharmaceutical Co., Ltd, Xi'an, 710043, China;
    3. Xi'an Medical University, Xi'an 710021, China

Received date: 2021-08-17

  Online published: 2024-06-24

Abstract

Objective: To establish the HPLC fingerprint of Zhengxin Jiangzhi tablets for its quality evaluation. Methods: The separation was performed on a Kromasil C18 column (4.6 mm×150 mm, 3.5 μm) with acetonitrile-0.1% formic acid solution as mobile phase. The flow rate was 0.8 mL·min-1. The detection wavelength was 254 nm and the column temperature was 25 ℃. The similarity evaluation of 10 batches of Zhengxin Jiangzhi tablets was conducted by “Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System (2012 edition)”. The common peaks were identified by Q-Exactive Orbitrap HRMS and the reference substance. Results: The fingerprint of Zhengxin Jiangzhi tablets was established and a total of 32 common peaks were marked. The similarities between the 10 batches of Zhengxin Jiangzhi tablets and the control fingerprint were greater than 0.99 after integrating all the 32 common peaks, while the similarities between the 10 batches of Zhengxin Jiangzhi tablets and the control fingerprints were greater than 0.95 after removing the rutin integral. A total of 26 common peaks were identified by Q-Exactive Orbitrap HRMS. By comparing with reference substance, 13 of the identified peaks by HRMS were further confirmed as 3'-hydroxy puerarin, puerarin, 3'-methoxy puerarin and daidzein, rutin, luteolin-7-O-glucuronide, hesperidin, daidzein, salvianolic acid A, quercetin, isorhamnetin, aurantio-obtusin, emodin. The adscription of the 32 common peaks was analyzed by comparing the Zhengxin Jiangzhi tablets sample with each single herb and negative sample, and the 32 common peaks covered all the 8 herbs in the prescription. Conclusion: The established method is simple, stable and specific, which will lay a foundation for the quality evaluation and substance basis study of Zhengxin Jiangzhi tablets.

Cite this article

CUI Xiao-min, HE Fang, JIN Yue-ti, REN Hui, HU Jing, QU Tong, LI Ning, CHEN Zhi-yong . Establishment of fingerprint of Zhengxin Jiangzhi tablets and the Q-Exactive Orbitrap HRMS analysis of its common peaks*[J]. Chinese Journal of Pharmaceutical Analysis, 2022 , 42(8) : 1440 -1447 . DOI: 10.16155/j.0254-1793.2022.08.18

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