Safety Monitoring

Establishment of a detection method on PCR-nucleic acid test strip to identify the authenticity of Gekko gecko*

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  • 1. School of Medical Technology, Beihua Universsity, Jilin 132013, China;
    2. School of Basic Medicine, Beihua University, Jilin 132013, China;
    3. Innovation Center for Detection on DNA Fingerprint of Traditional Chinese Medicine, Jilin 132013, China;
    4. Jilin Guoan Pharmaceutical Co., Ltd., Jilin 132013, China

Received date: 2023-02-06

  Online published: 2024-06-24

Abstract

Objective: To establish a PCR-nucleic acid test strip method to identify the authenticity of Gekko gecko, which is a precious Chinese medicinal herb in China,. Methods: The genomic DNA of Gekko gecko and its adulterants was extracted by alkaline denaturation method. With cytochrome C oxidase subunit I (CoI) as the target gene, specific primers were designed to determine the appropriate PCR-nucleic acid test strip reaction system and reaction conditions. Results: In this experiment, the qualitative analysis of Gekko gecko was carried out by PCR-nucleic acid test strip. The authentic Gekko gecko showed two bands on the PCR-nucleic acid test strip, while the counterfeit Gekko gecko and negative control both showed one band. Conclusion: The PCR-nucleic acid test strip detection method can achieve the visual detection of Gekko gecko in a short time, with wide application range, simple operation and accurate results, which can provide technical support for the identification of Gekko gecko DNA molecules.

Cite this article

CHI Kai-yue, MA Yu-he, MA Qiu-he, LIU Yu, LI Tao, LIU Xin-yue, GAO Li-jun, MU Run-hong, LI Ming-cheng, XIA Wei, QU Yong-mei . Establishment of a detection method on PCR-nucleic acid test strip to identify the authenticity of Gekko gecko*[J]. Chinese Journal of Pharmaceutical Analysis, 2023 , 43(9) : 1484 -1493 . DOI: 10.16155/j.0254-1793.2023.09.05

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