Metabolism Analysis

Pharmacokinetics of active components in Jinhong tablets in normal and superficial gastritis rats*

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  • 1. Department of Pharmacy, Beijing Health Vocational College, Beijing 101101, China;
    2. Department of Pharmacy, Beijing Friendship Hospital, Capital Medical University, Beijing100050, China;
    3. SCIEX China, Beijing 100015, China

Received date: 2022-12-08

  Online published: 2024-06-24

Abstract

Objective: To establish an UPLC-MS/MS method to determine the blood concentration of the main active components at different time points after oral administration, and to compare the difference in pharmacokinetics characteristics between normal rats and chronic superficial gastritis model rats and to identity the chemical components in Jinhong tablets based on UPLC-Q-TOF-MS/MS technology. Methods: A rat model of chronic superficial gastritis was established. Six rats in the normal group and six rats in the model group were given Jinhong tablets suspension (200 mg·kg-1) by gavage. Blood was taken from the inner canthus. The contents of corydaline, tetrahydropalmatine and shikimic acid in rat plasma samples were determined by UPLC-MS/MS, and the main pharmacokinetic parameters were calculated by DAS 2.0 software. Results: 54 chemical components were identified in the Jinhong tablets. The linear relationships between corydaline, tetrahydropalmatine, and shikimic acid were good within their respective ranges (r>0.991 2). The intra day and inter day precision of the three components ranges from 0.86% to 7.8%. The extraction recovery rate ranges from 77.9% to 110.6%, and the matrix effect ranges from 92.2% to 106.2%. The RSD of stability tests at room temperature for 12 hours, 4 ℃ refrigeration for 24 hours, and -80 ℃ freeze-thaw for 3 times were all less than 8.6%, meeting the requirements for biological sample analysis. Main pharmacokinetic parameters: The Cmax and AUC0-t of corydaline in the model group rats were higher than those in the normal group (P<0.01, P<0.05), while the Cmax and AUC0-t of tetrahydropalmatine were also higher than those in the normal group (P<0.01, P<0.01). The Cmax and AUC0-t of shikimic acid were lower than those in the normal group, while T1/2 and MRT were longer than those in the normal group. Conclusion: The pharmacokinetic behavior of Jinhong tablets in normal rats and chronic superficial gastritis model rats was different, which was due to the changes in the internal environment caused by pathological conditions, and the changes in the absorption and metabolism of effective ingredients of drugs.

Cite this article

HAO Jing-jing, GUO Ying-yu, HU Hai-yan, ZHANG Xuan-ling, CHEN Jun-miao . Pharmacokinetics of active components in Jinhong tablets in normal and superficial gastritis rats*[J]. Chinese Journal of Pharmaceutical Analysis, 2023 , 43(9) : 1565 -1573 . DOI: 10.16155/j.0254-1793.2023.09.14

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