Ingredient Analysis

Simultaneous determination of monosaccharides and oligosaccharides in Dendrobii Officinalis Caulis by HPLC-CAD*

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  • 1. Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, The Ministry of Education Key Laboratory for Standardization of Chinese Medicines, The State Administration of Traditional Chinese Medicine Key Laboratory for New Resources and Quality Evaluation of Chinese Medicine, Shanghai R&D Center for Standardization of Chinese Medicines, Shanghai 201203, China;
    2. Jinling Pharmaceutical Co., Ltd., Nanjing 210009, China

Received date: 2022-12-19

  Online published: 2024-06-24

Abstract

Objective: To develop a high performance liquid chromatography-charged aerosol detector (HPLC-CAD) method for simultaneous determination of ten monosaccharides and oligosaccharides in Dendrobii Officinalis Caulis. Methods: The components were separated on a Waters XBridge BEH Amide column (250 mm×4.6 mm, 3.5 μm) with acetonitrile and 0.1% triethylamine aqueous solution as the mobile phase by gradient elution at a flow rate of 1.0 mL·min-1. The column temperature was 40 ℃. Results: The ten carbohydrate components in Dendrobii Officinalis Caulis, including three monosaccharides components fructose, D-mannose and D-glucose, and oligosaccharide components sucrose, maltose and maltooligosaccharides maltotriose, maltotetraose, maltopentaose, maltohexaose and maltoheptaose, had good resolution with a good linear relationship within the linear range. The correlation coefficients were all above 0.999. The average recoveries were 98.2%-100.5% with RSD within 0.62%-3.0%. The LODs of above ten carbohydrate components were 0.014, 0.008, 0.026, 0.009, 0.008, 0.016, 0.035, 0.016, 0.016 and 0.017 μg·g-1, respectively. The RSDs of precision, stability and repeatability were less than 3.0%. The content ranges of fructose, D-mannose, D-glucose, sucrose, maltose, maltotriose, maltotetraose, maltopentaose, maltohexaose and maltoheptaose were 0.05-11.92 mg·g-1, 0.01-2.82 mg·g-1, 0.07-16.49 mg·g-1, 0.38-23.14 mg·g-1, 0.02-3.17 mg·g-1, 0-4.08 mg·g-1, 0-3.78 mg·g-1, 0-2.30 mg·g-1, 0-7.53 mg·g-1 and 0-6.02 mg·g-1, respectively, in 18 batches of Dendrobii Officinalis Caulis. The distribution and contents of 10 carbohydrate components in Dendrobii Officinalis Caulis had a certain correlation with the cultivation area. Conclusion: The established method is rapid, accurate, reproducible and simple, and can be used for simultaneous determination of monosaccharide and oligosaccharide components in Dendrobii Officinalis Caulis.

Cite this article

XU Shuang-shuang, WU Si-jia, LU Wen-xu, LI Jian, WANG Zheng-tao, XU Hong . Simultaneous determination of monosaccharides and oligosaccharides in Dendrobii Officinalis Caulis by HPLC-CAD*[J]. Chinese Journal of Pharmaceutical Analysis, 2023 , 43(8) : 1302 -1311 . DOI: 10.16155/j.0254-1793.2023.08.05

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