Objective: To establish a method for the determination of p-cymene and characteristic chromatogram of Jinghua Weikang gelatin capsules by gas chromatography. Methods: Agilent DB-17 capillary column(30 m×0.32 mm, 0.25 μm,the stationary phase was filled with 50% phenyl-methyl polysiloxane)was adopted. The temperature program was as follows: initial temperature: 60 ℃, increased to 80 ℃ at a rate of 1.5 ℃·min-1, and increased to 200 ℃ at a rate of 60 ℃·min-1. The temperature of FID detector and the inlet was both set at 250 ℃. The content of p-cymene in Jinghua Weikang gelatin capsule was determined by GC, and the GC characteristic chromatogram of Jinghua Weikang gelatin capsule was established. Results: The methodology of the assay and characteristic chromatogram were verified well. The GC fingerprints of 20 batches of Jinghua Weikang gelatin capsules were all more than 0.90 in similarity and ten common peaks were identified. p-cymene showed good linear relationship in the range of 0.049 4-2.470 0 μg. The average recovery rate was 97.8%. The contents of the above component ranged from 4.3 mg to 5.6 mg per capsule. Conclusion: The method is convenient, accurate and reliable, and can be used to establish the quality standard and evaluate the quality of Jinghua Weikang gelatin capsules.
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