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Experimental study on rapid identification of antler authenticity and its species detection reagents*

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  • 1. School of Medical Technology, Beihua University, Jilin 132013, China;
    2. School of Basic Medicine, Beihua University, Jilin 132013, China

Received date: 2023-04-23

  Online published: 2024-06-24

Abstract

Objective: To establish a duplex PCR detection system for rapid identification of pilose antler, red deer antler and common counterfeit reindeer antler according to the location and number of target bands on agarose gel electrophoresis. Methods: The genomic DNA of velvet antler samples was extracted by alkali denaturation method. The Cytb and COI genes of deer animals were used as target sequences, and Premier 3.0 was used to design specific identification primers (Cy-1, COI-1) based on SNP sites. The PCR reaction system and conditions were explored and optimized. At the same time, the accuracy of the test results was verified by cloning and sequencing. Results: The established duplex PCR amplification system showed that there were two amplified bands at 408 bp and 146 bp in the electrophoresis map of velvet antler, one band at 146 bp in velvet antler, and no amplified band in counterfeit velvet antler, which was consistent with the results of plasmid cloning and sequencing. The detection results of commercial antler samples were consistent with the identification results. Conclusion: The duplex PCR detection system constructed in this experiment is simple and rapid, and the detection reagent results are accurate and stable. It is of great practical value to realize one-step identification of velvet antler, red deer antler and their common counterfeits at the molecular level.

Cite this article

XU Ning, MA Yu-he, MA Qiu-he, AI Jin-xia, MU Run-hong, GAO Li-jun, XIA Wei . Experimental study on rapid identification of antler authenticity and its species detection reagents*[J]. Chinese Journal of Pharmaceutical Analysis, 2023 , 43(8) : 1435 -1445 . DOI: 10.16155/j.0254-1793.2023.08.21

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