Ingredient Analysis

Simultaneous determination of content of 23 components in Zuojin pills based on UPLC-MS/MS*

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  • 1. School of Pharmacy, Binzhou Medical University, Yantai 264003, China;
    2. Shandong Xianglong Pharmaceutical Research Institute Co., Ltd., Yantai 264000, China

Received date: 2023-06-25

  Online published: 2024-06-24

Abstract

Objective: To establish an UPLC-MS/MS method for the simultaneous determination of 13 components of Coptis chinensis Franch., including berberine, coptisine, epiberberine, jatrorrihizine, columbamine, palmatine, 8-oxo-epiberberine, berberrubine, magnoflorine, 8-oxocoptisine, dehydrocorydaline, protocatechuic acid and 5-O-feruloylquinic acid, and 10 components from Euodia rutaecarpa (Juss.) Benth, including evodiamine, rutaecarpine, dehydroevodiamine, dihydroevocarpine, evocarpine, 14-formyldihydroxyrutaecarpine, 1-methyl-2-undecenyl-4(1H)-quinolone, 1-methyl-2-nonyl-4(1H)-quinolone, skimmianine and diosiminin in Zuojin pills. Methods: The 80% methanol extract of Zuojin pills was analyzed on an ACQUITY UPLC BEH Shield RP 18 column (100 mm×2.1 mm, 1.7 μm), with acetonitrile-water containing 0.1% formic acid as mobile phase at a flow rate of 0.4 mL·min-1. The data was acquired by electrospray ionization source in multiple reaction monitoring mode. Results: 23 components showed good linear relationships within their respective ranges (r>0.999 0) and the average recoveries were 95.8%-104.0%. And the precision, repeatability and stability all met requirements. These 23 components were all determined in Zuojin pills, and the contents varied greatly. The content of berberine in Zuojin pills extracted by ethanol was the highest of 172.0 mg·g-1, while skimmianine was the lowest of 0.021 64 mg·g-1. Conclusion: The accurate, simple and stable method can be applied for the quality control and pharmacodynamic material basis study of Zuojin pills.

Cite this article

ZHANG Jia-ying, YIN Yi, XU Qian-qian, CHE Xiao-qing, ZHAO Juan-juan . Simultaneous determination of content of 23 components in Zuojin pills based on UPLC-MS/MS*[J]. Chinese Journal of Pharmaceutical Analysis, 2023 , 43(7) : 1110 -1119 . DOI: 10.16155/j.0254-1793.2023.07.03

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