Ingredient Analysis

Simultaneous determination of six kinds of components in Saposhnikoviae Radix by QAMS

Expand
  • 1. Taian Testing Institute for Food and Drug Control, Taian 271000, China;
    2. Shandong Center for Food and Drug Evaluation & Inspection, Jinan 250000, China

Received date: 2023-06-20

  Online published: 2024-06-24

Abstract

Objective: To establish quantitative analysis of multi-components to a single-marker(QAMS) method for determining contents of six ingredients(prim-O-glucosylcimifugin, cimifugin, 5-O-methylvisammioside, sec-O-glucosylhamaudol, psoralen and imperatorin) in Saposhnikoviae Radix and to verify the accuracy and feasibility of the method. Methods: A stable HPLC method was established and Agilent ZORBAX SB-C18(250 mm×4.6 mm,5 μm) column was adopted, the mobile phase consisted of acetonitrile(A)-0.02% phosphoric acid(B) in a gradient mode. The flow rate was 1.0 mL·min-1, the column temperature was set at 30 ℃, and the detection wavelength was set at 210 nm. QAMS method was established and validated for Saposhnikoviae Radix. Cimifugin, 5-O-methylvisammioside, sec-O-glucosylhamaudol, psoralen and imperatorin were selected an analytes while prim-O-glucosylcimifugin was chosen as internal reference substance to evaluate the quality. The relative correction factors(RCFs) of prim-O-glucosylcimifugin to the other five active ingredients were calculated. The method was evaluated by the comparison of quantity between external standard method(ESM) and QAMS method. Results: The contents of six active ingredients in 12 batches of Saposhnikoviae Radix determined by QAMS method had no significantly different with external standard method. Prim-O-glucosylcimifugin, cimifugin, 5-O-methylvisammioside, sec-O-glucosylhamaudol, psoralen, imperatorin showed good linear relationship in the ranges of 0.21%-0.49%, 0.060%-0.16%, 0.20%-0.41%, 0.11%-0.26%, 0.029%-0.093%, 0.066%-0.14%, respectively. Conclusion: The determination results of prim-O-glucosylcimifugin, cimifugin, 5-O-methylvisammioside, sec-O-glucosylhamaudol, psoralen and imperatorin in Saposhnikoviae Radix by QAMS is accurate and feasible, which can be used for the quality control of Saposhnikoviae Radix.

Cite this article

ZHANG Jun-peng, ZHUANG Hui, LIU Bin, ZHOU Hai-yan . Simultaneous determination of six kinds of components in Saposhnikoviae Radix by QAMS[J]. Chinese Journal of Pharmaceutical Analysis, 2023 , 43(7) : 1163 -1171 . DOI: 10.16155/j.0254-1793.2023.07.09

References

[1] 中华人民共和国药典 2020年版. Vol Ⅰ[S].2020: 156
ChP 2020. Vol Ⅰ[S].2020: 156
[2] 李阳, 王旭, 李壮壮, 等. 防风化学成分分离鉴定[J].中国实验方剂学杂志, 2017, 23(15):60
LI Y, WANG X, LI ZZ, et al. Isolate and identify chemical constituents of methylene chloride and ethyl acetate extract of Saposhnikoviae Radix[J].Chin J Exp Tradit Med Form, 2017, 23(15):60
[3] 辛国, 李鑫, 黄晓巍, 等. 防风化学成分及药理作用[J].吉林中医药, 2018, 38(11):1323
XIN G, LI X, HUANG XW, et al. Research progress on chemical constituents and pharmacological action of Radix Sileris[J].Jilin J Chin Med, 2018, 38(11):1323
[4] 刘双利, 姜程曦, 赵岩, 等. 防风化学成分及其药理作用研究进展[J].中草药, 2017, 48(10):2146
LIU SL, JIANG CX, ZHAO Y, et al. Advance in study on chemical constituents of Saposhnikovia divaricata and their pharmacological effects[J].Chin Tradit Herb Drug, 2017, 48(10):2146
[5] 王丽丽, 廖强, 欧妮, 等. 一测多评法测定防风中4种色原酮类成分[J].中国药师, 2018, 21(1):96
WANG LL, LIAO Q, OU N, et al. Determination of four chromones in Saposhnikoviae Radix by multi-components with single marker[J].China Pharm, 2018, 21(1):96
[6] 徐容. 关防风HPLC特征指纹图谱研究及4个色原酮类成分一测多评法分析研究[J].药物分析杂志, 2019, 39(3):458
XU R. Study on fingerprint and multi-component quantitation by one marker method of Radix Saposhnikoviae from northeast[J].Chin J Pharm Anal, 2019, 39(3):458
[7] 赵博, 杨鑫宝, 杨秀伟, 等. HPLC法同时测定防风中6个主要成分的含量[J].药物分析杂志, 2013, 33(3):382
ZHAO B, YANG XB, YANG XW, et al. Simultaneous determination of six major constituents in the roots of Saposhnikovia divaricata by HPLC[J].Chin J Pharm Anal, 2013, 33(3):382
[8] 杨晓琴, 任红燕. 高效液相色谱法同时测定防风中4种主要成分含量[J].中国药业, 2017, 26(20):37
YANG XQ, REN HY. Content determination of four main components in Saposhnikovia divaricata by HPLC[J].China Pharm, 2017, 26(20):37
[9] 胡紫艳, 冯振斌, 蒋健. HPLC测定不同产地防风中4种有效成分的含量[J].中国执业药师, 2016, 13(11):31
HU ZY, FENG ZB, JIANG J. Determination of four components in Saposhnikovia divarcata from different habitats by HPLC[J].China Licen Pharm, 2016, 13(11):31
[10] 韩旭, 彭金咏, 许丽娜, 等. HPLC测定防风通圣丸中7种有效成分的含量[J].中国药学杂志, 2014, 49(2):156
HAN X, PENG JY, XU LN, et al. Simultaneous determination of seven components in Fangfengtongsheng pills by HPLC[J].Chin Pharm J, 2014, 49(2):156
[11] 刘加元, 张建超. HPLC法同时测定保儿宁颗粒中10种成分[J].中成药, 2020, 42(7):1709
LIU JY, ZHANG JC. Simultaneous determination of ten constituents in Baoerning granules by HPLC[J].Chin Tradit Pat Med, 2020, 42(7):1709
[12] 曲颂扬, 关开, 周慧, 等. HPLC法同时测定外感风寒颗粒中10种成分[J].中成药, 2020, 42(4):853
QU SY, GUAN K, ZHOU H, et al. Simultaneous determination of ten constituents in Waiganfenghan granules by HPLC[J].Chin Tradit Pat Med, 2020, 42(4):382
[13] 廖小芳, 毕晓黎, 胥爱丽, 等. UPLC法同时测定玉屏风颗粒中9种成分[J].中成药, 2019, 41(8):1778
LIAO XF, BI XL, XU AL, et al. Simultaneous determination of nine constituents in Yupingfeng granules by UPLC[J].Chin Tradit Pat Med, 2019, 41(8):1778
[14] 邹胜泽, 罗哲婵, 张雁, 等. 一测多评法同时测定正天丸中6种指标性成分[J].中国现代应用药学, 2019, 36(3):331
ZOU SZ, LUO ZC, ZHANG Y, et al. Determination of six index components in Zhengtian pills by quantitative analysis of multi-components by single marker[J].Chin J Mod Appl Pharm, 2019, 36(3):331
[15] 唐林, 肖望重, 黄莉, 等. 一测多评法同时测定清解颗粒中3种成分[J].中成药, 2019, 41(9):2052
TANG L, XIAO WZ, HUANG L, et al. Simultaneous determination of three constituents in Qingjie granules by QAMS[J].Chin Tradit Pat Med, 2019, 41(9):2052
[16] 许庆, 安艳苏, 毕秋左. 一测多评法同时测定栀榆洗剂[J].中国药业, 2020, 29(15):58
XU Q, AN YS, BI QZ. Content determination of four components in Zhiyu lotion by QAMS[J].China Pharm, 2020, 29(15):58
[17] 魏长勇, 徐敏灵. 替代对照品内加入法测定复方甘草片中的甘草苷和甘草酸[J].华西药学杂志, 2020, 35(4):442
WEI CY, XU ML. Determination of liquiritin and glycyrrhizic acid in the compound liquorice tablets by the internal addition method with substitute reference substance[J].West China J Pharm Sci, 2020, 35(4):442
[18] 郭立强, 李熙, 刘谦, 等. 一测多评法比较不同产地甘草中6种有效成分含量[J].中国现代应用药学, 2019, 36(24):3051
GUO LQ, LI X, LIU Q, et al. Simultaneous quantitative analysis of six active ingredients in Glycyrrhizae Radix et Rhizoma from different habitats by QAMS[J].Chin J Mod Appl Pharm, 2019, 36(24):3051
[19] 吴娇芬, 崔慧芳, 张为, 等. 一测多评法测定芒果叶化学成分的含量[J].中药材, 2020, 43(2):398
WU JF, CUI HF, ZHANG W, et al. Determination of chemical constituents in Mango leaves by QAMS[J].J Chin Med Mater, 2020, 43(2):398
[20] 李晓青, 田雅娟, 杜鹃, 等. 一测多评法测定人参花中7种人参皂苷含量[J].中草药, 2019, 50(24):6120
LI XQ, TIAN YJ, DU J, et al. Determination of seven saponins in flower buds of Panax ginseng by quantitative analysis of multi-components by single marker[J].Chin Tradit Herb Drugs, 2019, 50(24):6120
[21] 匡艳辉, 廖丰蕴, 李雯衫, 等. 一测多评法测定细辛中5种成分含量[J].中药材, 2019, 42(9):2091
KUANG YH, LIAO FY, LI WS, et al. Determination of five components in Asarum by QAMS[J].J Chin Med Mater, 2019, 42(9):2091
[22] 秦晋颖, 谢晓琳, 赵元, 等. 一测多评法同时测定艾纳香油中5种成分[J].中成药, 2020, 42(2):381
QIN JY, XIE XL, ZHAO Y, et al. Simultaneous determination of five constituents in the oil from balsamifera by QAMS[J].Chin Tradit Pat Med, 2020, 42(2):381
[23] 谭丙寅, 吴明波, 袁芳杰. 一测多评法同时测定安中片中8种成分[J].中成药, 2019, 41(11):2581
TAN BY, WU MB, YUAN FJ. Simultaneous determination of eight constituents in Anzhong tablets by QAMS[J].Chin Tradit Pat Med, 2019,41(11):2581
[24] 许伟. 一测多评法同时测定宁神定志丸中8种成分[J].中成药, 2019, 41(11):2586
XU W. Simultaneous determination of eight constituents in Ningshendingzhi pills by QAMS[J].Chin Tradit Pat Med, 2019, 41(11):2586
Outlines

/