Objective: To investigate the metabonomic characteristics of arachidonic acid(AA) in the prostate of benign prostatic hyperplasia (BPH) rats after the intervention of the flavonoids extract of Anemarrhenae Rhizoma. Methods: Shim-PACK XR-ODS C18(75 mm×3.0 mm, 2.2 μm) column was performed in UPLC assay. The mobile phase A was 0.05% formic acid aqueous solution(concentrated ammonia test solution adjusted to pH 3.3)and mobile phase B was methanol-acetonitrile (20∶80, v/v). Gradient elution was performed at the flow rate of 0.3 mL·min-1, the column temperature was 40 ℃, and the injection volume was 10 μL. Negative ionscanning and multiple reaction monitoring(MRM) mode was employed formass spectrometry.The prostate tissues of rats in the sham group, BPH model group, flavonoids group and positive control group were collected and detected, and a total of eleven substances[including arachidonic acid (AA), 5-hydroxyeicosatetraenoic acid (5-HETE), 8-hydroxyeicosatetraenoic acid (8-HETE), 11-hydroxyeicosatetraenoic acid (11-HETE), 12-hydroxyeicosatetraenoic acid (12-HETE), 15-hydroxyeicosatetraenoic acid (15-HETE), leukotriene B4 (LTB4), prostaglandin D2 (PGD2), prostaglandin E2 (PGE2), prostaglandin H2 (PGH2), thromboxane B2 (TXB2)]were quantitatively analyzed by ultra-fast liquid chromatography tandem mass spectrometry(UFLC-MS/MS). Results: The objects to be measured could be well detected and its calibration curves were good linear in the linear range. The extraction recovery rates of 11 components to be tested in tissue samples at high, medium, and low concentrations were all greater than 75.7%, with matrix effects ranging from 85.5% to 106.3%, accuracy RSD<11.2%, and intra day and inter day precision RSD<11.0% and 9.5%, respectively.The selectivity, accuracy and precision, recovery, matrix effects, and stability of the method met the requirements. Studies had shown that the concentrations of AA and its metabolites in BPH model group rat prostate were significant higher than those in sham group rats.The flavonoids extract of Anemarrhenae Rhizoma could change the development process of BPH and effectively down-regulate the concentrations of AA and its metabolites in the BPH rat prostate.Conclusion: The flavonoids extract of Anemarrhenae Rhizoma can regulate AA metabolism through two pathway of lipoxygenase(LOX) and cyclooxygenase(COX), thereby inhibiting the inflammatory process occurring in BPH.
CAO Xiao-tong, JIANG Shu-qin, KONG Wei-gui, SHANG Ying, LIAO Jun, JU Tao, DAI Rong-hua
. Effect of flavonoids extract of Anemarrhenae Rhizoma on the metabolism of arachidonic acid in benign prostatic hyperplasia rats based on targeted metabonomics*[J]. Chinese Journal of Pharmaceutical Analysis, 2023
, 43(7)
: 1195
-1204
.
DOI: 10.16155/j.0254-1793.2023.07.13
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