Objective: To establish a quantitative analysis of multi-components by single-marker(QAMS) method for simultaneous determination of nine saponins in Paridis Rhizoma, in order to provide basis for studying its quality standards. Methods: The analysis was performed on Agilent Zorbax SB C18 column(4.6 mm×250 mm, 5 μm), with mobile phase composed of acetonitrile-water at a flow rate of 1.0 mL·min-1 in gradient elution mode. The column temperature was maintained at 20 ℃,and the detection wavelengths were set at 203 nm. Paridis Rhizoma reference extract was used to locate chromatographic peaks, Chonglou saponin Ⅶ was selected as the internal standard, and the relative correlation factors(RCFs) of Chonglou saponin D, Chonglou saponin H, Chonglou saponin Ⅵ, Chonglou saponin Ⅱ, dioscin, gracillin, Chonglou saponin Ⅰ and Chonglou saponin Ⅴ were determined by HPLC, and the feasibility and accuracy of QAMS method were verified. Results: The standard curves of nine saponins in Paridis Rhizoma had good linear relationship in the ranges of the tested concentrations(R2>0.999 9). The precision, stability and repeatability complied with the requirements of methodology. The RCFs of Chonglou saponin D, Chonglou saponin H, Chonglou saponin Ⅵ, Chonglou saponin Ⅱ, dioscin, gracillin, Chonglou saponin Ⅰ, and Chonglou saponin Ⅴ were 0.906 3, 0.929 3, 0.725 6, 0.989 0, 0.876 8, 0.883 9, 0.822 1 and 0.810 2, respectively, which had good repeatability in different experimental conditions. The total content of nine saponins in 45 batches of Paris polyphylla Smith var. yunnanensis was 2.211%, and in 35 batches of Paris polyphylla Smith var. chinensis was 2.965%. Conclusion: The QAMS method is feasible and accurate for the determination of the nine saponins and is beneficial to the quality control of in Paridis Rhizoma.
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