Objective: To establish a method for charateristic chromatogram of Longgu Anshen liquor and to determine the contents of spinosin, hypericin, epimedin A, epimedin B, epimedin C, icariin, psoralen, isopsoralen, baohuosideⅠand schisandra A, and provide reference its quality control. Methods: Agilent Technologies Zorbax SB-C18 column (4.6 mm×250 mm, 5 μm) was used, with the mobile phase comprising of acetonitrile-water flowing at 1.0 mL·min-1 in a gradient elution manner,the column temperature was 30 ℃,and the detection wavelength was set at 270 nm. Based on similarity evaluation, cluster analysis (CA), principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA), the fingerprints of 12 batches of Longgu Anshen liquor were analyzed and evaluated, and the 10 index components identified were quantitatively determined. Results: The HPLC fingerprint of Longgu Anshen liquor was constructed with 15 common chromatographic peaks, The similarities of each batches of samples were all above 0.9 comparing with the reference. There were some differences in the quality of 12 batches of Longgu Anshen liquor samples, and five key components were screened out, which were spinolin, hyperin, epimedin C, icariin and psoralen.The 10 constituents showed good linear relationship(r≥0.999 6), whose content ranges were 10.59-18.59 μg·mL-1,48.51-61.11 μg·mL-1,13.74-20.78 μg·mL-1,13.05-20.05μg·mL-1,231.82-271.29 μg·mL-1,113.77-148.98 μg·mL-1,60.89-80.58 μg·mL-1,58.90-71.68 μg·mL-1,17.91-21.17 μg·mL-1,64.09-76.34 μg·mL-1. Conclusion: The established charateristic chromatograms of Longgu Anshen liquor and the content determination method, simple accurate and reliable,and can be used for its quality control.
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