Objective: To establish HPLC fingerprint of Qinlian capsules and simultaneously determine the contents of seven components including paeoniflorin, coptisine hydrochloride, forsythoside A, berberine hydrochloride, palmatine hydrochloride, baicalin and glycyrrhizic acid. Methods: The HPLC analysis was performed on a column of Agela Durashell RP C18(250 mm×4.6 mm, 5 μm) with gradient elution of methanol-0.05 mol·L-1 potassium dihydrogen phosphate aqueous solution containing 0.05% phosphoric acid at a flow rate of 1.0 mL·min-1. The detection wavelengths were 230 nm for paeoniflorin and glycyrrhizic acid, 280 nm for baicalin, 346 nm for coptidine hydrochloride, forsythin A, berberine hydrochloride and palmatine hydrochloride, and the column temperature was 35 ℃. Results: The HPLC fingerprint of Qinlian capsules was stablished and 20 common peaks were found. The similarities of 20 batches of samples were above 0.97. The seven compounds were well separated under the chromatographic conditions. The RSD values for precision and repeatability were all less than 2.0% and the test solution was stable in 48 h. Seven compounds showed good linearities and wide linear ranges, whose recoveries were 96.5%-101.9% with the RSDs of 0.30%-1.9%. The contents of paeoniflorin, coptisine hydrochloride, forsythoside A, berberine hydrochloride, palmatine hydrochloride, baicalin and glycyrrhizic acid in 20 batches of Qinlian capsules were 6.95-14.37, 0.27-2.46, 3.47-8.43, 9.82-17.80, 1.90-3.55, 13.12-25.90 and 1.64-4.40 mg·g-1, respectively. Conclusion: The method of HPLC fingerprint analysis and multi-index components determination is simple and accurate, which can be used for the quality control of Qinlian capsules.
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