Objective: To study the protective mechanism of naringin in total flavonoids of Rhizoma Drynariae on learning, memory and cognitive ability of scopolamine-induced memory damage model mice from the perspective of metabonomics. Methods: Three-month-old male Kunming mice were randomly divided into blank group, model group and treatment group according to their body weight. A model of memory damage was constructed using scopolamine. The effects of naringin on learning and memory ability in mice were observed by object recognition test and water maze test. Then mouse blood samples were detected for metabolite profiling analysis and the changes of blood metabolite analysis. Results: The behavioral experimental results showed that the learning and memory abilities of the mice after administration of the drug were significantly improved as compared with those in the model group. The metabolomics research results showed that the metabolic profiles of mouse blood showed a significant callback trend after administration of drug, and 21 differential metabolites were identified, of which, the levels of L-acetylcarnitine and citric acid significantly increased after administration, while the levels of proline, tryptophan, lysophosphatidyl choline(14∶0), lysophosphatidyl choline(15∶0), deoxycytidine and 4-aminophenol significantly decreased after administration, involving taurine and hypotaurine metabolism, tryptophan metabolism, arginine and proline metabolism, vitamin B6 metabolism, tyrosine metabolism and citric acid cycle. Conclusion: Naringin can improve the learning and memory ability of scopolamine model mice. The protective effect of naringin may be related to improving energy metabolism, reducing β-amyloid protein (Aβ) production, reducing Tau hyperphosphorylation, promoting synaptic plasticity, improving acetylcholine system disorders, anti-oxidative stress and anti-cells.
LIN Xue, ZHAO Chen-yu, DONG Rui, ZHANG Yi-bo, YAO Rui-min, GUO Ting-xiu, ZHANG Ning, XU Yan-ming
. Hematological metabonomics study of naringin improving learning cognitive ability of scopolamine model mice*[J]. Chinese Journal of Pharmaceutical Analysis, 2022
, 42(2)
: 263
-270
.
DOI: 10.16155/j.0254-1793.2022.02.10
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