Objective: To investigate the effect of LncRNA FAS-AS1 on cisplatin (Cis) resistance of glioma cells and its related molecular mechanism. Methods: Differentially expressed LncRNA FAS-AS1 was detected by RT-PCR between U87 and U251 cells and between U87/Cis and U251/Cis cells. MTT assay, CCK-8 assay and flow cytometry assay were used to detect the proliferation, activity and apoptosis of U87/Cis and U251/Cis cells overexpressing LncRNA FAS-AS1 in a certain concentration of cisplatin. The autophagy capacity of U87/Cis and U251/Cis cells was analyzed by immunofluorescence, and the autophagy and apoptosis-related proteins were further verified by Western blot and RT-PCR. The changes of autophagy in U87/Cis and U251/Cis cells after over-expression of LncRNA FAS-AS1 were further analyzed. Results: The expression of LncRNA FAS-AS1 was significantly low in U87/Cis and U251/Cis cell lines. The over-expression of LncRNA FAS-AS1 in U87/Cis and U251/Cis cell lines resulted in no significant change in cell proliferation, but decreased cell viability and significantly increased cell apoptosis. With the increase of cisplatin concentration, the expression levels of autophagy and apoptosis-related proteins in U87/Cis and U251/Cis cells increased gradually. The expression levels of autophagy related proteins in U87/Cis and U251/Cis cells were decreased after the overexpression of LncRNA FAS-AS1. Conclusion: LncRNA FAS-AS1 promotes the sensitivity of glioma cells to cisplatin by inhibiting excessive autophagy.
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