Objective: To analyze the main volatile constituents of Virginia Cedar essential oils from the market, and to explore its pharmacological activity in vitro. Methods: GC-MS method was adopted. The determination was performed on capillary column of Agilent HP-5MS (250 μm×30 m,0.25 μm), and high purity helium was used as carrier gas. The injection volume was 0.2 μL, the split ratio was 10∶1, the column flow was 1.0 mL·min-1 and the inlet temperature was 220 ℃. The ion source was EI, and its temperature was 230 ℃. The ionization energy was 70 eV, the mass scan range was m/z 12-550 and the solvent delay was 3 minutes. The relative contents of each component were determined by area normalization method. The size of inhibition zone was determined by Oxford cup method and the antibacterial activity to the Staphylococcus aureus, Escherichia coli and Candida albicans in vitro was evaluated. Lipopolysaccharide (LPS) stimulated RAW246.7 cells inflammation model was established to investigate its effect on the inflammatory factor TNF-α expression level of RAW264.7 in vitro. Its cytotoxic activity in vitro was tested on the HT-29 cells by methyl thiazolyl tetrazolium (MTT) method. Results: 21 compounds were identified in the essential oils sample of Virginia Cedar, accounting for 95.67% of the total peak area, and the main components were terpenes and alcohols, such as Cedrol (26.58%), α-Cedrene (24.05%), and cis-Thujopsene (23.46%). Virginia Cedar essential oils inhibited the tumor cell HT-29 proliferation in a dose dependent manner, and significantly increased the expression of TNF-α. But the sensitivity of the essential oils to the three strains were not high. Conclusions: The essential oils of Virginia Cedar is rich in ingredients, promoting the polarization of RAW264.7 cells model induced by LPS to M1 type and showing better anti-tumor activity to HT-29 cells.
XI Zu-wei, WANG Yu-jia, CHEN Jin-hu, WANG Meng-ru, LI Ting, LIU Heng
. GC-MS analysis and in vitro study on pharmacological activity of Virginia Cedar essential oils*[J]. Chinese Journal of Pharmaceutical Analysis, 2022
, 42(2)
: 335
-341
.
DOI: 10.16155/j.0254-1793.2022.02.19
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