Objective: To establish an ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for the simultaneous determination of twenty six components(magnolol, honokiol, magnolflorine, magnocurarine, magnoloside A, magnoloside B, hesperidin, nobiletin, tangeretin, sinensetin, narirutin, naringenin, ginsenoside Rb1, 6-gingerol, liquiritin, glycyrrhizic acid, liquiritigenin, isoliquiritigenin, isoliquiritin, isoliquiritin apioside, verbascoside, isoacteoside, chlorogenic acid, neochlorogenic acid, caffeic acid and rutin)in Yangwei decoction. Methods: The separation was performed on a Shim-pack GIST C18 column (2.1 mm×100 mm,2.0 μm) with a mobile phase consisting of acetonitrile and 0.1% acetic acid-1 mmol·L-1 ammonium acetate water solution with gradient elution at a flow rate of 0.3 mL·min-1. The column temperature was 30 ℃, the injection volume was 1 μL and the analysis time was 18.0 min. The detection was carried out by electrospray ionization (ESI). Positive and negative electrospray ionization was performed in multiple reaction monitoring (MRM) mode. Results: All of the analytes showed good linearity (r≥0.996 9) in the tested ranges. The precision, repeatability and stability of the method were good for the twenty six components. The average recoveries were in the range of 96.5%~104.2% with relative standard deviations RSD≤4.0%. The contents of magnolol, honokiol, magnolflorine, magnocurarine, magnoloside A, magnoloside B, hesperidin, nobiletin, tangeretin, sinensetin, narirutin, naringenin, ginsenoside Rb1, 6-gingerol, liquiritin, glycyrrhizic acid, liquiritigenin, isoliquiritigenin, isoliquiritin, isoliquiritin apioside, verbascoside, isoacteoside, chlorogenic acid, neochlorogenic acid, caffeic acid and rutin in 6 batches of Yangwei decoction were 2 814.12-2 951.41, 2 551.48-2 675.66, 288.12-312.36, 439.88-494.60, 452.00-500.74, 329.76-358.68, 5 995.57-6 283.13, 70.16-73.81, 22.95-24.21, 5.56-6.39, 997.32-1 077.03, 6.17-6.81, 643.88-674.16, 994.16-1 063.20, 1 929.00-1 984.85, 2 356.80-2 518.96, 247.68-256.64, 38.02-43.18, 228.84-241.4, 227.17-234.87, 129.61-150.39, 28.97-31.90, 622.85-643.23, 526.05-542.73, 70.09-78.64 and 152.41-166.3 μg·g-1, respectively. Conclusion: The established method is accurate, stable and reproducible,and can provide a research method for the quality control of Yangwei decoction.
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