Objective: To observe the effect of orientin on arrhythmia rats and its influence on electrocardiogram (ECG) through Notch pathway. Methods: A rat model of arrhythmia was established by sublingual injection of aconitine and randomly divided into a model group, low dose of orientin group (0.75 mg·kg-1), middle dose of orientin group (1.5 mg·kg-1), high dose of orientin group (3 mg·kg-1), and a control group. ECG after intervention was monitored, and the heart rate (HR), QT interval, PR interval, QRS complex time, and time of occurrence of premature ventricular contraction (PVC), ventricular tachycardia (VT), and ventricular fibrillation (VF), HE staining was compared to observe the pathological changes of myocardial tissue.TUNEL staining was used to observe the apoptosis of cardiomyocytes.The mRNA and protein expressions of Notch1, Notch intracellular domain (NICD), hairy and enhancer of split 1 (Hes1) in myocardial tissue were compared. Results: Compared with control group, HR were accelerated, QT interval and PR interval were prolonged, QRS complex was widened, the apoptotic rate of cardiomyocytes was increased, mRNA and protein expressions of Notch1, NICD and Hes1 in myocardial tissue were decreased in model group (P<0.05). Compared with the model group, HR slowed down, QT interval, PR interval shortened, QRS complexes narrowed, the appearance time of PVC, VT, VF prolonged, the apoptotic rate of cardiomyocytes decreased,and the expression of Notch1, NICD, Hes1 mRNA and protein in myocardial tissue increased in each dose group (P<0.05). Among which the high dose of orientin group had the most obvious therapeutic effect (P<0.05). Conclusion: Orientin may prevent and treat arrhythmia by regulating Notch signaling pathway.
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