Objective: To establish an HPGPC-CAD method for the determination of molecular mass and molecular mass distribution of dextran 40,and to compare the result with the HPGPC-RID method. Methods: Molecular mass and molecular mass distribution of dextran 40 were determined by HPGPC with TSK G4000 PWXL column(300 mm×7.8 mm,10 μm), and the mobile phase for HPGPC-CAD was methanol-0.02 mol·L-1 ammonium formate (30∶70). And the mobile phase for HPGPC-RID was 0.71% sodium sulfate solution, containing 0.02% sodium azide), the results were handled with the WATERS GPC software. Results: HPGPC-CAD determination showed that the linear range was 2 700-300 600 (r=0.997 2),and the precision, stability, repeatability (n=6) were 0.32%, 0.23%, 0.81%, respectively. In comparison, HPGPC-RID determination showed that the linear range was 2 700-300 600 (r=0.995 4),and the precision, stability, repeatability (n=6) was 0.20%, 0.15%, and 0.26%, respectively. Conclusion: Both HPGPC-CAD and HPGPC-RID methods are stable and reliable, and can be used for the determination of molecular mass and molecular mass distribution of dextran 40.
KANG Bi, SHUAI Wei-wei, CHENG Dong
. Comparison of HPGPC-CAD and HPGPC-RID methods for determination of molecular mass and molecular mass distribution of dextran 40[J]. Chinese Journal of Pharmaceutical Analysis, 2021
, 41(11)
: 1954
-1959
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DOI: 10.16155/j.0254-1793.2021.11.13
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