Safety Monitoring

Rapid determination of 13 chemical drugs in antidiabetic health foods by HPLC-DAD*

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  • Wuhan Institute for Drug and Medical Device Control, Wuhan 430075, China

Received date: 2020-11-10

  Online published: 2024-06-24

Abstract

Objective: To develop and apply a method for the simultaneous determination of 13 drugs in antidiabe-tic health foods by HPLC-DAD. Methods: The samples were extracted by ultrasonic machine with methanol and the contents were determined by HPLC-DAD. The target analytes were separated on Inert Sustain C18 column (4.6 mm×250 mm, 5 μm) with a mobile phase consisting of 10 mmol·L-1 ammonium acetate and methanol by gradient elution at the flow rate of 1.0 mL·min-1 and the column temperature was 35 ℃. The detection wavelength was set at 235 nm for qualification and quantification analysis of 13 antidiabetic chemical drugs. Results: The calibration curves were linear within the ranges of corresponding concentration. The correlation coefficients were above 0.999 8 for all 13 antidiabetic chemical drugs. The recoveries were in the range of 78.6%-111.2% with RSD of 0.10%-4.5% at three spiked levels. In the 11 batches of samples, the hypoglycemic chemicals were found in 4 batches, and all the 4 batches contained more than one drugs, in which phenformin and glidenclamide were found in four samples, rosiglitazone was found in two samples, pioglitazone was found in one sample. Conclusion: The established method is simple, accurate, rapid and reproducible for qualification and quantification of 13 hypoglycemic chemicals in hypoglycemic health products, which can meet the daily testing requirements of the laboratory and has practical application significance.

Cite this article

QIN Juan, XIE Bai-yan . Rapid determination of 13 chemical drugs in antidiabetic health foods by HPLC-DAD*[J]. Chinese Journal of Pharmaceutical Analysis, 2021 , 41(11) : 1990 -1999 . DOI: 10.16155/j.0254-1793.2021.11.17

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