Review & Monography

Factors influencing sample identification and quantification in proteomics*

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  • College of Pharmacy, Nanjing University of Traditional Chinese Medicine, Nanjing 210023, China

Received date: 2021-03-29

  Online published: 2024-06-25

Abstract

Protein is the key component in the regulation of biological structure and functions, which is of great complexity. Mass spectrometry (MS)-based proteomics has become the main method for protein qualitative and quantitative research and showed great advantages in the screening of many disease-related biomarkers and identification of drug targets. In this paper, the MS-based bottom-up proteomics analysis was briefly summarized, besides, the effects of sample preparation, liquid chromatographic separation, mass spectrum acquisition strategies and the setting of relative parameters on protein identification and quantification were introduced in detail. The purpose of this review is to optimize the precision, accuracy and coverage of protein qualitative and quantitative results in clinical samples and provide a reference for subsequent drug design and discovery.

Cite this article

LI Bing-xu, CHEN Wu-yue, WANG Jiao-jiao, PENG Shan, LÜ Xiang, MA Hong-yue, ZHOU Jing . Factors influencing sample identification and quantification in proteomics*[J]. Chinese Journal of Pharmaceutical Analysis, 2021 , 41(12) : 2049 -2054 . DOI: 10.16155/j.0254-1793.2021.12.01

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