Objective: To investigate the cytotoxic effect of the active components of Vernonia anthelmintica on human normal hepatocytes (LO2) and human normal skin tissue fibroblasts (HFF) cells, so as to provide experimental basis for its clinical use. Methods: Normal liver cells LO2 HFF were adopted.The IC50 values of LO2 and HFF cells under the action of three different active components were measured by drug cytotoxicity test. Three different cytotoxicity tests, including CCK-8, total protein concentration and LDH release were used to evaluate the cytotoxicity of the active components of Vernonia anthelmintica on to LO2 and HFF cells. Results: Considerably reduction in the survival rate at different active components of Vernonia anthelmintica concentrations were observed on LO2、HFF cell lines. The IC50 of butin, butein, isorhamnetin on the LO2 cell lines reached 67.537,157.383 and 214.043 μmol·L-1,respectively. Compared with the control group, 50, 100(P<0.05), 150, 200, 250, 300, 400(P<0.01) μmol·L-1 experimental group could significantly reduce the protein contents of LO2 cells, while increase cell mortality and LDH release levels;when the concentration of isorhamnetin was increased to 200, 250(P<0.05), 300,400(P<0.01) μmol·L-1, the protein contents of LO2 cells could be significantly reduced, while cell mortality and LDH release levels were increased. Isorhamnetin at lower concentrations had no statistical significance on the survival rate of LO2 cells; the concentration was less than 150 μmol·L-1 in the butein group, and there was no effect on the survival rate of LO2 cells. When the concentration reaches 150,200 (P<0.05), 250, 300, 400 (P<0.01) μmol·L-1, the protein contents of LO2 cells were significantly decreased,while the cell mortality and LDH release levels were increased.The IC50 of butin、 butein、isorhamnetin on the HFF cell lines reached to 165.54, 260.57 and 178.04 μmol·L-1,respectively. Compared with the control group, butin 100, 150, 200 (P<0.05), 250, 300, 400 (P<0.01) μmol·L-1 experimental group could significantly reduce the protein content of HFF cells. While the cell mortality and LDH release levels were increased;When the concentration of isorhamnetin was increased to 150 (P<0.05), 200, 250, 300, 400 (P<0.01) μmol·L-1, the protein content of HFF cells could be significantly reduced, while the cell mortality and LDH release levels were increased. Isorhamnetin at lower concentrations had no statistical significance on the survival rate of HFF cells; the concentration was less than 150 μmol·L-1 in the butein group, and there was no effect on the survival rate of HFF cells. When the concentration reached 200, 250, 300 (P<0.05), 400 (P<0.01) μmol·L-1, the protein contents of HFF cells were significantly decreased, while the cell mortality and LDH release were increased. Conclusion: The active components of Vernoniaanthelminticum, butin, butein, isorhamnetin are cytotoxic to LO2 and HFF, suggesting that the side effects of Vernoniaanthelminticus could not be ignored in clinical administration.
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