Objective: To use an ultra performance liquid chromatography- tandem mass spectrometry (UPLC-MS/MS) method for the simultaneous determination of the contents of 11 chemical components (gallic acid, methyl gallate, catechin, brervifolincaboxylic acid, corilagin, eraniin, ellagic acid, isoquercitrin, pentagalloyl glucose, astragalin and oleanic acid) in Yi nationality medicine G. strictipes. Methods: The separation was performed on a Shim-pack GIST C18 column(100 mm×2.1 mm, 2.0 μm) with a mobile phase consisting of acetonitrile (A) and 0.1% acetic acid water solution (B) in gradient elution. The flow rate was 0.4 mL·min-1, the column temperature was set at 30 ℃ and the injection volume was 1 μL. Electrospray ionization (ESI) was used for negative ion detection in multiple reaction monitoring (MRM) mode. Results: The measured 11 chemical components had good linear relationship within the range of measured concentration with correlation coefficients(r) all above 0.999 4. The precision, repeatability and stability were good with RSDs≤4.6%. The average recoveries varied between 98.7% and 109.7% with RSDs≤3.3%. The contents of gallic acid, methyl gallate, catechin, brervifolincaboxylic acid, corilagin, eraniin, ellagic acid, isoquercitrin, pentagalloyl glucose, astragalin and oleanic acid in 15 batches of samples were 1 084.0-3 533.2, 62.4-345.0, 206.8-641.2, 741.0-1 483.4, 2 502.0-4 073.8, 15 826.4-68 268.6, 0.3-18.1, 42.4-128.0, 2 349.5-7 670.9, 3.9-49.7 and 1.6-29.6 μg·g-1, respectively. Conclusion: The method is accurate and sensitive, with good stability and reproducibility, and can be applied to the quality assessment of G. strictipes. It also can provide reference for the quality control and clinical application of G. strictipes used for the quality control and subsequent development and research of the medicinal materials of G. strictipes.
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