Objective: To establish a QAMS method for the simultaneous determination of four flavonoids including isovitexin, isovitexin-3”-O-glucopyranoside, isoscoparin, chrysoeriol 6-C-β-D-glucopyranosyl-(1→3)-β-glucopyranoside in Folium Isatidis. Methods: The analysis of this drug was performed on a 30 ℃ thermostatic ACE Excel 5 C18 column(250 mm×4.6 mm, 5 μm), with the mobile phase comprising of acetonitrile-0.1% phosphoric acid(13.5∶86.5) at a flow rate of 1.0 mL·min-1. And the detection wavelength was set at 269 nm. Isovitexin was used as an internal standard to calculate the relative correction factors of the other three constituents, after which the content determination was performed. Results: Four constituents showed good linear relationships within their own ranges(r≥0.999 5), whose average recoveries were 97.0%-100.3% with the RSDs of 0.71%-1.2%. The contents of isovitexin, isovitexin-3”-O-glucopyranoside, isoscoparin, chrysoeriol 6-C-β-D-glucopyranosyl-(1→3)-β-glucopyranoside, in nine batches of samples were 1.005-2.067 mg·g-1, 0.134-1.876 mg·g-1, 0.545-1.519 mg·g-1 and 0.167-2.587 mg·g-1. The results obtained by QAMS and external standard method were approximate. Conclusion: This simple, reliable and reproducible method can be used for the quality control of Folium Isatidis.
[1] 杨立国, 王琪, 苏都那布其, 等. 菘蓝属植物化学成分及药理作用研究进展[J]. 中国现代应用药学, 2021, 38(16):2039
YANG LG, WANG Q, SUDU NBQ, et al. Research progress on chemical constituents and pharmacological effects of Isatis[J]. Chin J Mod Appl Pharm, 2021, 38(16):2039
[2] 中华人民共和国药典 2020年版. 一部[S]. 2020: 21
ChP 2020. Vol Ⅰ[S]. 2020: 21
[3] 闫峻, 顾娟, 冯硕, 等. 大青叶化学成分及抗氧化活性研究[J]. 质谱学报, 2019, 40(4):381
YAN J, GU J, FENG S, et al. Study of chemical constituents and antioxidant activity of Isatidis Folium[J]. J Chin Mass Spectrom Soc, 2019, 40(4):381
[4] 胡永金, 乔金玲, 朱仁俊, 等. 紫草与大青叶提取物体外抑菌效果研究[J]. 安徽农业科学, 2010, 38(9):4565
HU YJ, QIAO JL, ZHU RJ, et al. Study on antibacterial activity of Arnebia euchroma (Royle) Johnst and Isatis indigotica Fort extracts in vitro[J]. J Anhui Agric Sci, 2010, 38(9):4565
[5] 喻淑庆, 陈湘漪, 余凌. 大青叶提取物抗单纯疱疹病毒Ⅱ型的体外实验研究[J]. 医药导报, 2008, 27(4):394
YU SQ, CHEN XY, YU L. Anti-herpes effects of the extract from indigowoad leaf in vitro[J]. Her Med, 2008, 27(4):394
[6] 罗芬. 大青叶水提物抗甲型流感病毒活性研究[J]. 新乡医学院学报, 2017, 34(10):881
LUO F. Antiviral activity of Folium Isatidis aqueous extract on influenza avirus[J]. J Xinxiang Med Univ, 2017, 34(10):881
[7] YU YP, GONG DD, ZHU Y, et al. Quality consistency evaluation of Isatidis Folium combined with equal weight quantified ratio fingerprint method and determination of antioxidant activity[J]. J Chromatogr B, 2018, 1095(31):149
[8] 方建国, 施春阳, 汤杰, 等. 大青叶抗内毒素活性部位筛选[J]. 中草药, 2004, 35(1):64
FANG JG, SHI CY, TAN J, et al. Screening of active fraction of antiendotoxin from Folium Isatidis[J]. Chin Tradit Herb Drugs, 2004, 35(1):64
[9] NAM S, SCUTO A, YANG F, et al. Indirubin derivatives induce apoptosis of chronic myelogenous leukemia cells involving inhibition of Stat 5 signaling[J]. Mol Oncol, 2012, 6(3):276
[10] 夏小艳, 刘可春, 王思锋, 等. 大青叶中靛玉红的抗血管生成活性研究[J]. 中国药学杂志, 2010, 45(3):187
XIA XY, LIU KC, WANG SF, et al. Studies on anti-angiogenic activity of indirubin from Folium Isatidis[J]. Chin Pharm J, 2010, 45(3):187
[11] 刘富康, 方士元, 曲映红. 大青叶粗黄酮提取及其抑菌性研究[J]. 山东化工, 2018, 47(21):34
LIU FK, FANG SY, QU YH. Study on extraction and antibacterial activity of crude flavonoids from Folium Isatidis[J]. Shandong Chem Ind, 2018, 47(21):34
[12] 张海涛, 刘祺凤, 田璐阳, 等. 超声波辅助提取大青叶中总黄酮及其抗氧化性研究[J]. 湖北农业科学, 2014, 53(9):2124
ZHANG HT, LIU QF, TIAN LY, et al. Ultrasound－assisted extraction and antioxidative activities of total flavonoids from Folium Isatidis[J]. Hubei Agric Sci, 2014, 53(9):2124
[13] 赵震毅, 李国斌. 异金雀花素等5种大青叶黄酮的同时检测[J]. 食品工业, 2017, 38(10):283
ZHAO ZY, LI GB. Determination of five flavonoidsin different Folium Isatidis extracts by using HPLC[J]. Food Ind, 2017, 38(10):283
[14] 周洪旭, 张毅, 杨新勇, 等. 一测多评法测定川射干中8个异黄酮类成分的含量[J]. 药物分析杂志, 2022, 42(6):1072
ZHOU HX, ZHANG Y,YANG XY, et al. Simultaneous determination of 8 isoflavones in Iris tectorum Maxim by quantitative analysis of multi-components with single marker[J]. Chin J Pharm Anal, 2022, 42(6):1072
[15] 万红才, 徐作刚, 段萍, 等. 一测多评法测定乌蕨中5个黄酮成分含量[J]. 药物分析杂志, 2021, 41(12):2211
WAN HC, XU ZG, DUAN P, et al. Determination of 5 flavonoids in Sphenomers chinensis by quantitative analysis of multi-components by single marker[J]. Chin J Pharm Anal, 2021, 41(12):2211
[16] 陈欢, 马玲, 苏晓娟, 等. 枸杞子中4种类胡萝卜素成分含量测定一测多评法的建立[J].中国药房, 2022, 33(8):957
CHEN H, MA L, SU XJ, et al. Establishment of quantitative analysis of multi-components by singer marker for content determination of 4 carotenoids in Lycium barbarum[J]. China Pharm, 2022, 33(8):957
[17] 耿若愚, 居博伟, 杨建华, 等. 一测多评法测定新疆假龙胆中3种环烯醚萜苷[J]. 中成药, 2022, 44(3):829
GENG RY, JU BW, YANG JH, et al. Simultaneous determination of three iridoid glycosides in Gentianella turkestanerum by QAMS[J]. Chin Tradit Pat Med, 2022, 44(3):829
