Objective: To establish a method with high accuracy and applicability for the content determination of colloidal bismuth pectin capsules. Methods: UV-Vis spectrophotometry used 10% ascorbic acid solution-20% potassium iodide solution-1 mol·L-1 nitric acid solution(2∶5∶1) as chromogenic agent, and the determination wavelength was 463 nm. Potentiometric titration was carried out using photometric electrode as indicator electrode at 590 nm, with xylenol orange as an indicator and 0.05 mol·L-1 EDTA as titration. The wavelength was measured at 223.1 nm by atomic absorption spectrophotometry(AAS). Results: The good linear relationships were found in the range of 2-20 μg·mL-1,30-70 mg and 5-30 μg·mL-1 for UV-Vis spectrophotometry, potentiometric titration and AAS, respectively. The average recoveries were 100.0% (RSD=0.40%), 100.4% (RSD=0.49%) and 100.3%(RSD=0.51%). Eighteen batches of samples were determined by the above three methods, the content ranges were 97.1%-104.8%, 97.4%-105.0% and 97.5%-104.8%, respectively. The three established methods could accurately determine the content of colloidal pectin bismuth capsules, and the results showed no significant difference. Conclusion: Considering the universality of the instrument and the sensitivity of method, UV-Vis spectrophotometry is more suitable for the content determination of colloidal bismuth pectin capsules.
CHEN Huan, HUANG Yu-xin, ZANG Ya-ni, SUN Ying, MA Ling, SHEN Xiao-hua, LIU Shu-ying
. Establishment and comparison of content determination methods for colloidal bismuth pectin capsules*[J]. Chinese Journal of Pharmaceutical Analysis, 2023
, 43(4)
: 589
-594
.
DOI: 10.16155/j.0254-1793.2023.04.07
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