Objective: To establish an ultra-high performance liquid chromatography-photodiode array detector(UPLC-PDA) method for the simultaneous determination of 8 nucleoside components(guanine, uridine, adenine, inosine, guanosine, 2’-deoxyguanosine, β-thymidine, and adenosine) in American ginseng, and to determine the nucleosides in different parts of American ginseng, so as to provide a scientific basis for the quality control and evaluation of American ginseng. Methods: Acquity UPLC HSS T3 column (100 mm×2.1 mm, 1.8 μm) was adopted, the mobile phase was water-acetonitrile with gradient elution at the flow rate of 0.3 mL·min-1, the column temperature was 30 ℃, the detection wavelength was 260 nm, and the injection volume was 2 μL. Results: The 8 nucleosides had good linearity in the range of 1.00-200.00 μg·mL-1 with correlation coefficients all above 0.999 6, the average spiked recoveries were 98.1%-99.9%, and the RSD range was 1.2%-2.6%. The sensitivity, accuracy and precision were all in line with the requirement. The contents of nucleoside in samples from the rhizome, fibrous root and main lateral roots of American ginseng from Weihai, Shandong Province were 2 556.18, 1 829.93 and 1 100.67 mg·kg-1, respectively, and the results were significantly different (P＜0.05). Conclusion: The method can be appied in determination of nucleosides in American ginseng and the differentiation of different parts, which offers preliminary evaluation of the quality of American ginseng.
[1] 中华人民共和国药典 2020年版. 一部[S].2020: 136
ChP 2020. Vol Ⅰ[S].2020: 136
[2] 任多多, 邵紫君, 刘松鑫, 等. 西洋参多糖对克林霉素磷酸酯诱导的抗生素相关性腹泻的改善作用[J].食品工业科技, 2021, 42(12):354
REN DD, SHAO ZJ, LIU SX, et al. Ameliorative effect of Panax quinquefolius polysaccharides on antibiotic associated diarrhea induced by clindamycin phosphate[J].Sci Technol Food Ind, 2021, 42(12):354
[3] 丁涛, 尚智, 温富春, 等. 西洋参茎叶总皂甙对小鼠腹腔巨噬细胞免疫功能作用的研究[J].长春中医药大学学报, 2007, 23(6):14
DING T, SHANG Z, WEN FC, et al. Effects of total saponins from American ginseng stems and leaves on the immune function of mouse peritoneal macrophages[J].J Changchun Univ Chin Med, 2007, 23(6):14
[4] LI ZM, ZHAO LJ, CHEN JB, et al. Ginsenoside Rk1 alleviates LPS-induced depression-like behavior in mice by promoting BDNF and suppressing the neuroinflammatory response[J].Biochem Biophys Res Commun, 2020, 530(4):658
[5] KING L, MURPHY LL. Role of cyclin inhibitor protein p21 in the inhibition of HCT116 human colon cancer cell proliferation by American ginseng (Panax quinquefolius) and its constituents[J].Phytomedicine, 2010, 17(3-4):261
[6] WANG XX, WANG CM, WANG JM, et al. Pseudoginsenoside F11(PF11) exerts anti-neuroinflammatory effects on LPS-activated microglial cells by inhibiting TLR4-mediated TAK1/IKK/NF-κB, MAPKs and Akt signaling pathways[J].Neuropharmacology, 2014, 79: 642
[7] SHAO ZH, XIE JT, VANDEN HOEK TL, et al. Antioxidant effects of American ginseng berry extract in cardiomyocytes exposed to acute oxidant stress[J].Biochim Biophys Acta, 2004, 1670(3):165
[8] KIM MH, LEE J, JUNG S, et al. The involvement of ginseng berry extract in blood flow via regulation of blood coagulation in rats fed a high-fat diet[J].J Ginseng Res, 2017, 41(2):120
[9] 王燕华, 孙印石, 张磊, 等. 鹿茸不同炮制品化学成分的对比分析[J].中国中药杂志, 2018, 43(6):1145
WANG YH, SUN YS, ZHANG L, et al. Comparative analysis of chemical components in different processed deer antler products[J].China J Chin Mater Med, 2018, 43(6):1145
[10] CHEN P, LI W, LI Q, et al. Identification and quantification of nucleosides and nucleobases in Geosaurus and Leech by hydrophilic-interaction chromatography[J].Talanta, 2011, 85(3):1634
[11] 张雪梅, 杨丰庆, 夏之宁. 食品中核苷类成分的药理作用研究进展[J].食品科学, 2012, 33(9):277
ZHANF XM, YANG FQ, XIA ZN. Research progress on pharmacological effects of nucleosides in food[J].Food Sci, 2012, 33(9):277
[12] WEI C, PRABHU B, DAVID GP. Comparison of ginsenoside components of various tissues of New Zealand forest-grown Asian ginseng (Panax ginseng) and American ginseng (Panax quinquefolium L.)[J].Biomolecules, 2020, 10(372):113
[13] 刘丽敏. 西洋参中L-精氨酸及其衍生物的研究[D].长春: 吉林农业大学, 2008
LIU LM. Study on L-Arginine and Its Derivatives in American Ginseng[D].Changchun: Jilin Agricultural University, 2008
[14] 崔德深. 西洋参[M].第3版. 北京: 科学出版社, 1984: 165
CUI DS. Panax quinquefolium L.[M].3rd Ed. Beijing: Science Press, 1984: 165
[15] 孙印石, 王燕华, 王玉方, 等. UPLC法测定不同加工方式梅花鹿鹿茸中的核苷类成分[J].中草药, 2018, 49(4):840
SUN YS, WANG YH, WANG YF, et al. Simultaneous determination of nucleosides and nucleobases in Cervi Cornu Pantotrichum of sika deer(Cervus nippon) with different processing methods by UPLC[J].Chin Tradit Herb Drugs, 2018, 49(4):840
[16] 郑友兰, 张崇禧, 李向高. 人参和西洋参芦头与根的化学成分比较分析[J].中国中药杂志, 1989, 24(6):332
ZHENG YL, ZHANG CX, LI XG. Comparative analysis of chemical constituents in the head and root of Panax ginseng and American ginseng[J].China J Chin Mater Med, 1989, 24(6):332
[17] QU CL, BAI YP, JIN XQ, et al. Study on ginsenosides indifferent parts and ages of Panax quinquefolius L.[J].Food Chem, 2008, 115(1):340
[18] XIE JT, MEHENDALE SR, WANG AB, et al. American ginseng leaf: ginsenoside analysis and hypoglycemic activity[J].Pharmacol Res, 2004, 49(2):113
[19] WANG CZ, WU J, MCENTEE E, et al. Saponins composition in American ginseng leaf and berry assayed by high-performance liquid chromatography[J].J Agric Food Chem, 2006, 54(6):2261
[20] 刘俊文, 徐美利, 徐冰, 等. 西洋参不同部位皂苷成分研究[J].天津中医药, 2019, 36(7):715
LIU JW, XU ML, XU B, et al. Study on the components of saponins in different parts of American ginseng[J].Tianjin J Tradit Chin Med, 2019, 36(7):715
[21] 李乐, 张春阁, 李小沛, 等. 不同时期人参、西洋参根中单体皂苷Rg1、Re、Rb1、Rb2含量的比较[J].特产研究, 2017, 2(8):38
LI L, ZHANG CG, LI XP, et al. Comparison of the contents of monomer saponins Rg1, Re, Rb1 and Rb2 in ginseng and American ginseng roots in different periods[J].Spec Wild Econ Anim Plant Res, 2017, 2(8):38
[22] 张福泉, 袁建文. 西洋参生长规律的观察[J].中药材, 1988, 6(11):5
ZHANG FQ, YUAN JW. Observation on growth law of American gainseng[J].J Chin Med Mater, 1988, 6(11):5
[23] 陆国胜. 西洋参真空冷冻干燥工艺研究[J].食品研究与开发, 2018, 39(14):115
LU GS. Study on vacuum freeze-drying process of American ginseng[J].Food Res Dev, 2018, 39(14):115
[24] 魏晓雨, 田义新, 赵智灵, 等. 不同产地西洋参种质遗传多样性的RAPD和ISSR分析[J].中草药, 2014, 45(21):3153
WEI XY, TIAN YX, ZHAO ZL, et al. RAPD and ISSR analysis of genetic diversity of American ginseng germplasm from different origins[J].Chin Tradit Herb Drugs, 2014, 45(21):3153
[25] GAFNER S, BERGERON C, MCCOLLOM MM, et al. Evaluation of the efficiency of three different solvent systems to extract triterpene saponins from roots of Panax quinquefolius using high-performance liquid chromatography[J].J Agric Food Chem, 2004, 52(6):1546
[26] ZHANG SQ, CHEN RZ, WU H, et al. Ginsenoside extraction from Panax quinquefolium L.(American ginseng) root by using ultrahigh pressure[J].J Pharm Biomed Anal, 2006, 41(1):57
