Objective: To establish a method for determination of two tomix impurities(epichlorhydrin and glycidol) in choline glycerophosphate by GC-MS. Methods: Sample was dissolved and diluted with methanol. The determination was performed on ZB-WAXplusTM column (30 m×0.25 mm, 0.25 μm), the column temperature was programmed temperature(initially heated at 30 ℃ for 1 min, rose to 220 ℃ at a speed of 30 ℃·min-1 remaining 5 min). using high purity He as carrier gas, the injector temperature was 200 ℃, splitless, the sample volume was 1 μL, The ion source was electrospray ion source, the ion source temperature was 200 ℃, the ionization energy was 70 eV, Transmission line temperature was 250 ℃, the scan mode was selective ion monitoring(SIM), the ion mass charge ratio(m/z) of epichlorhydrin were 49, 57, 62, the m/z of glycidol were 31, 43, 44, the solvent delay was 3 min, qualitified by the external reference method. Results: Epichlorhydrin showed a good linear relationship(r=0.999 7) in the concentration range of 29.86-746.48 ng·mL-1, the LOD was 19.91 ng·mL-1, the LOQ was 29.86 ng·mL-1, RSDs of precision and repeatability were less than 10%. The average recovery (n=9) was 93.9% with RSD of 5.1%. Glycidol showed a good linear relationship(r=0.999 4) in the concentration range of 172.91-922.18 ng·mL-1,LOD was 115.27 ng·mL-1, the LOQ was 172.91 ng·mL-1, RSDs of precision and repeatability were less than 10%. The average recovery (n=9) was 91.4% with RSD of 3.1%. In the 4 batches of samples tested, the contents of epichlorhydrin and glycidol were negtive. Conclusion: This method is simple, efficient and repeatable, it can be used to determine the contents of epichlorhydrin and glycidol in L-α-glycerophosphorylcholine.
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