Objective: To establish an HPLC method for the simultaneous determination of gallic acid and dehydrodiisoeugenol in Sanwei Tanxiangtang powder. Methods: Ultimate XB-C18 liquid chromatographic column (250 mm×4.6 mm,5 μm) was adopted. The mobile phase was composed of 0.05% phosphoric acid aqueous solution (A) - methanol (B) with gradient elution(0-60 min, 10%B→100%B). The total flow rate was 1.0 mL·min-1, the detection wavelength was 270 nm, the column temperature was 25 ℃, and the injection volume was 10 μL. Results: Gallic acid and dehydrodiisoeugenol showed good linear relationships (r=0.999 9) in the range of 1.20-72.00 μg·mL-1 and 3.20-192.00 μg·mL-1 respectively. The average recoveries of gallic acid and dehydrodiisoeugenol in six samples were 100.2% (RSD=1.8%) and 102.0% (RSD=1.2%) respectively. And the precision (RSD=0.93%, RSD=0.46%), repeatability (RSD=1.2%, RSD=0.76%) and stability (RSD=1.0%, RSD=1.1%) met the requirements. The contents of gallic acid in two batches of samples were 0.194 mg·g-1 and 0.218 mg·g-1, respectively, and the contents of dehydroisoiseugenol were 0.686 mg·g-1 and 0.724 mg·g-1, respectively. Conclusion: The method is verified by the method of prescription and can be used for the quality evaluation of Tibetan medicine Sanwei Tanxiangtang powder.
GE Ya-qian, XIE He-bing, NIMA Ci-ren, BAIMA Dan-zeng
. Simultaneous determination of index components in Tibetan medicine Sanwei Tanxiangtang powder by HPLC*[J]. Chinese Journal of Pharmaceutical Analysis, 2023
, 43(5)
: 748
-753
.
DOI: 10.16155/j.0254-1793.2023.05.04
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