Objective: To establish a method for the quantitative analysis of methamphetamine and adulterants in seized methamphetamine crystals and tablets by low field LF-qNMR and high field HF-qNMR. Methods: 1H quantitative nuclear magnetic resonance (1H qNMR) was used to determine the content of methamphetamine and adulterants in seized methamphetamine crystal samples and methamphetamine tablets by Magritek SpinsolveTM 80ULTRA benchtop NMR spectrometer and Bruker Avance Ⅲ 400 MHz NMR spectrometer. Methanol-d4 and D2O were used as solvent. Maleic acid was used as internal standard. Results: The coefficients of determination (r) were all greater than 0.99. Bland-Altman plots showed that the differences between two measurement methods are within the 95% limits of agreement for 93% samples. The contents of methamphetamine and adulterants obtained by LF-qNMR spectrometer and HF-qNMR spectrometer can statistically be considered equal. Conclusion: The LF-qNMR and HF-qNMR quantitative method developed in this work applied to methamphetamine quantification is validated with good results. The sample preparation is simple and test time is short. This method is suitable for fast quantitative analysis of methamphetamine and adulterants.
SONG Chun-hui, LIU Cui-mei, JIA Wei, HUA Zhen-dong, WANG You-mei, DI Bin
. Quantitative analysis of methamphetamine and adulterants by low-field and high-field NMR*[J]. Chinese Journal of Pharmaceutical Analysis, 2023
, 43(5)
: 793
-803
.
DOI: 10.16155/j.0254-1793.2023.05.09
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