Ingredient Analysis

Determination of five glucaric acids and one malic acid in Leonurus japonicus by HPLC

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  • 1. Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China;
    2. Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China

Revised date: 2023-01-17

  Online published: 2024-06-25

Abstract

Objective: To establish an HPLC method for the determination of five glucaric acids and a malic acid in Leonurus japonicus. Methods: The separation was performed on an Agilent Poroshell 120 EC-C18 column (100 mm×4.6 mm, 2.7 μm), using 0.1% formic acid and acetonitrile as mobile phase with gradient elution. The column temperature was 30 ℃. The detection wavelength was 280 nm. The flow rate was 0.8 mL·min-1. Results: The six compounds (2-syringylglucaric acid, 2-feruloylglucaric acid, 2,4-disyringylglucaric acid, 3,4-disyringylglucaric acid, 2-syringylmalic acid, and 2-feruloyl-4-syringylglucaric acid) had good linear relationship within the certain range of concentrations (r≥0.999 8). The average spiked recoveries were between 95.4% and 100.1%, with the RSD values less than 3.9%. The average contents of six compounds in 30 batches of Leonurus japonicus from different regions ranged from 0.027 8% to 0.235 3%, and the total contents of glucaric acids ranged from 0.524 8% to 1.459%. Conclusion: The glucaric acids, as important compounds in Leonurus japonicus, should not be ignored.

Cite this article

WANG Zhu-qin, LI Yu-jie, ZHANG Hong-yang, ZHANG Min, WANG Yue-rong, HU Ping . Determination of five glucaric acids and one malic acid in Leonurus japonicus by HPLC[J]. Chinese Journal of Pharmaceutical Analysis, 2023 , 43(6) : 957 -963 . DOI: 10.16155/j.0254-1793.2023.06.06

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