Objective: To establish an HPLC method for the determination of five glucaric acids and a malic acid in Leonurus japonicus. Methods: The separation was performed on an Agilent Poroshell 120 EC-C18 column (100 mm×4.6 mm, 2.7 μm), using 0.1% formic acid and acetonitrile as mobile phase with gradient elution. The column temperature was 30 ℃. The detection wavelength was 280 nm. The flow rate was 0.8 mL·min-1. Results: The six compounds (2-syringylglucaric acid, 2-feruloylglucaric acid, 2,4-disyringylglucaric acid, 3,4-disyringylglucaric acid, 2-syringylmalic acid, and 2-feruloyl-4-syringylglucaric acid) had good linear relationship within the certain range of concentrations (r≥0.999 8). The average spiked recoveries were between 95.4% and 100.1%, with the RSD values less than 3.9%. The average contents of six compounds in 30 batches of Leonurus japonicus from different regions ranged from 0.027 8% to 0.235 3%, and the total contents of glucaric acids ranged from 0.524 8% to 1.459%. Conclusion: The glucaric acids, as important compounds in Leonurus japonicus, should not be ignored.
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