Metabolism Analysis

Study on absorption promotion and synergistic effect of curcumin and trametenolic acid B*

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  • 1. Hubei Provincial Key Laboratory of Natural Products Research and Development, China Three Gorges University, Yichang 443002, China;
    2. Yichang Hospital of Traditional Chinese Medicine, Yichang 443008, China

Received date: 2022-10-31

  Online published: 2024-06-25

Abstract

Objective: To provide evidence for improving the bioavailability of curcumin (CUR) and trametenolic acid B (TAB) by observing the effect of CUR and TAB on absorption and anti-tumor activity. Methods: The growth of SGC7901 cells treated with CUR and TAB was evaluated by MTT assays. The accumulation of CUR and TAB in the SGC7901 cells was analyzed by high performance liquid chromatogram (HPLC). The effect of CUR combined with TAB on blood drug concentration in rats was determined by HPLC. Results: CUR (12 μg·mL-1) combined with TAB (40 μg·mL-1) inhibited the proliferation of SGC7901 cells, and the drug combination index <1, indicating that CUR and TAB had a synergistic effect. After CUR (24 μg·mL-1) was combined with TAB (80 μg·mL-1), the concentration of CUR in cells was 2.98 times of CUR alone, the concentration of TAB was 1.63 times of TAB alone. These results showed that CUR and TAB promoted the absorption of each other in vitro. After oral administration, the Cmax of CUR in rats was 6.48 μg·mL-1, and Cmax of CUR of the combined group was 14.74 μg·mL-1. The Cmax of TAB in rats was 16.77 μg·mL-1 after oral administration and and 48.67 μg·mL-1 after combined with TAB. Conclusion: The combination of CUR and TAB can promote absorption mutually, and synergistically inhibit the proliferation of SGC7901 cells.

Cite this article

YAN Hua-ling, WANG Jun-zhi, DENG Gai-gai, LEI Jing-peng, GAO Yuan-xi, CAO Hong-ye, SONG Yu, GUO Meng-jia, DENG Zhang-shuang . Study on absorption promotion and synergistic effect of curcumin and trametenolic acid B*[J]. Chinese Journal of Pharmaceutical Analysis, 2023 , 43(6) : 964 -974 . DOI: 10.16155/j.0254-1793.2023.06.07

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