Objective: To explore the mathematical model that can be used to guide the separation of alkaloids in Arecae Semen when ion-pair reagents are used as mobile phase additives. To explore the separation mechanism of this type of additives. Methods: High performance liquid chromatography was used to separate alkaloids in arecae semen. Ion-pair reagents were used as mobile phase additives. Two typical retention models:stoichiometric displacement model for retention of solute (SDM-R) and Snyder’s empirical formula were built. The effect of ion-pair reagents concentration, alkyl chain length, volume fraction of organic phase on retention behavior of components, retention process and mechanism were investigated. Results: When the ion-pair reagents reach a certain range of concentration as mobile phase additives, the concentration of ion-pair reagents and retention value of components complied with the linear relationship of SDM-R model. The volume fraction of the organic phase and the retention value of components complied with the linear relationship of Snyder’s empirical formula. Conclusion: The SDM-R model and the Snyder’s empirical formula can provide guidance for the liquid-phase separation of alkaloids in Arecae Semen with ion-pair reagents as additives. When ion-pair reagents were used as mobile phase additives, the separation of alkaloids was effected by ion-exchange and ion-pairing. The application of two models has great significance to the quality control of traditional Chinese medicine with alkaloids as the main active components.
SHI Peng-jie, WU Jia, XIN Zhen-qiang, QIAN Yong
. Influence of sodium alkyl sulfonate ion-pair reagents on retention behavior of alkaloids in Arecae Semen*[J]. Chinese Journal of Pharmaceutical Analysis, 2023
, 43(6)
: 975
-981
.
DOI: 10.16155/j.0254-1793.2023.06.08
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