Objective: To establish an HPLC characteristic fingerprints of Rubiae Radix et Rhizoma and Rubia Wallichiana Radix et Rhizoma(the basic sources are Rubia wallichiana Decne. and Rubia tibetica Hook.f.). The multivariate statistical analysis of Rubiae Radix et Rhizoma and Rubia Wallichiana Radix et Rhizoma was carried out to provide reference for the use and basic research of Rubia Wallichiana Radix et Rhizoma. Methods: The HPLC analysis was carried out on an ACE Excel 5 C18 column(250 mm×4.6 mm,5 μm) with acetonitrile-0.1% trifluoroacetic acid (adjusting pH 2.6 with ammonia water) was used as the mobile phase. The flow rate was 1.0 mL·min-1, the detecion wavelength was 270 nm and the columntemperature was 30 ℃. Chempattern chemometrics software was used for statistical analysis. Results: Similarity analysis showed that the characteristics of Rubiae Radix et Rhizoma,Rubia wallichiana Decne. and Rubia tibetica Hook.f. were obviously different. Cluster analysis clustered Rubiae Radix et Rhizoma and Rubia wallichiana Radix et Rhizoma into two groups, and Rubia wallichiana Decne. and Rubia tibetica Hook.f. were also clustered into two groups. The main components in the principal component analysis was the compound of chromatographic peak No.16 and Mollugin in Rubiae Radix et Rhizoma, the compound of chromatographic peak No.14 in Rubia wallichiana Decne., and the compound of chromatographic peak No.1 in Rubia tibetica Hook.f.,respectively. Conclusion: The established characteristic maps and evaluation criteria can be used to identify Rubiae Radix et Rhizoma and Rubia wallichiana Radix et Rhizoma.
WANG Jun, LIU Ya-rong, SONG Xia, ZHANG Fu-jun, YAN Feng
. Study on characteristic fingerprints of Rubiae Radix et Rhizoma and Rubia Wallichiana Radix et Rhizoma based on multivariate statistical analysis*[J]. Chinese Journal of Pharmaceutical Analysis, 2022
, 42(11)
: 2043
-2049
.
DOI: 10.16155/j.0254-1793.2022.11.22
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