Safety Monitoring

Determination of paraquat residues in 6 sample matrices by ultra performance liquid chromatograph-tandem mass spectrometry*

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  • 1. College of Food Science, South China Agricultural University, Guangzhou 510642, China;
    2. Guangzhou Institute of Food Inspection, Guangzhou 511400, China

Received date: 2022-09-27

  Online published: 2024-06-26

Abstract

To establish a method for the determination of paraquat residues in 6 sample matrices by ultra performance liquid chromatograph-tandem mass spectrometry. Methods:Samples were extracted by acetonitrile-formic acid water solution, purified by HLB solid-phase extraction column, separated by hydrophilic amino chromatography column and quantified by mass spectrometry electrospray positive ion mode ionization(ESI+) with quadrupole multi-reaction detection mode(MRM). The method compensated for matrix inhibition effect by corn, calf serum and pig urine with isotope internal standard quantitation. Paraquat was easy to be adsorbed and degraded in glassware, thus the glassware used in the whole experiment was replaced by plastic ware. Results: The calibration curve showed good linearity in the range of 0.2-5 ng·mL-1 with the correlation coefficient(r2) of 0.998 2, and the limit of detection(LOD) of the method reached 0.15 μg·kg-1 when the sampling volume was 10 g. The recoveries of paraquat from cabbages, pears, corn, rice, calf serum and pig urine showed that the recoveries were 79.2%-106%, and the RSDs were 0.077%-2.4% at addition levels of 1.2 μg·kg-1, 3.0 μg·kg-1 and 6.0 μg·kg-1. The determination results of paraquat in 23 batches of samples were all undetected by random sampling of 6 sample matrices from local farmers markets. Conclusion: The method is sensitive, convenient and versatile, and can be applied to the determination of paraquat residues in food, agricultural products, blood and urine.

Cite this article

WANG Cheng-long, LIU Jia, XU Zhen-lin, HUANG Xiao-qing, LIN Ze-shan, TAN Jin-ping, WANG Yu, WANG Hong . Determination of paraquat residues in 6 sample matrices by ultra performance liquid chromatograph-tandem mass spectrometry*[J]. Chinese Journal of Pharmaceutical Analysis, 2022 , 42(12) : 2155 -2162 . DOI: 10.16155/j.0254-1793.2022.12.12

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