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Simultaneous determination of five flavonoids in leaves of Ampelopsis grossedentata by HPLC-MS/MS*

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  • Wuhan Customs Technology Center, Wuhan 430050, China

Received date: 2022-10-01

  Online published: 2024-06-26

Abstract

Objective: To establish an HPLC-MS/MS method for simultaneous determination of five flavonoids in leaves of Ampelopsis grossedentata. Methods: The separation was performed on a CNW Athena C18-WP (100 mm×2.1 mm, 3 μm) column with gradient elution of methanol-0.1% formic acid aqueous solution at a flow rate of 0.3 mL·min-1 and a column temperature of 30 ℃. Negative ion detection was adopted with electrospray ion source and multiple reaction monitoring mode. Results: The linear ranges of myricetin, taxifolin, quercetin 3-rhamnoside, kaempferol and (-)-epicatechin gallat were 54.4-1 030 ng·mL-1, 28.4-1 000 ng·mL-1, 27.4-1 000 ng·mL-1, 24.8-998 ng·mL-1 and 30.6-1 010 ng·mL-1 (r>0.996 8), respectively. The RSDs of precision, stability and repeatability tests were below 5.0% (n=6), the average recoveries were 96.4%, 99.8%, 97.7%, 95.0% and 100.9% with RSDs of 4.7%, 1.4%, 1.1%, 4.3% and 3.0% (n=6), respectively. The average contents of the above 5 components in 4 samples were 0.825-1.7, 5.15-10.05, 0.34-1.95, 0-0.002 and 0.198-2.62 mg·g-1, respectively. Conclusion: The method is simple, rapid, accurate, sensitive and repeatable, which can be used for the determination of five flavonoids in leaves of Ampelopsis grossedentata.

Cite this article

YE Cheng, WANG Dong-peng, HUANG Ling-ling . Simultaneous determination of five flavonoids in leaves of Ampelopsis grossedentata by HPLC-MS/MS*[J]. Chinese Journal of Pharmaceutical Analysis, 2022 , 42(12) : 2195 -2201 . DOI: 10.16155/j.0254-1793.2022.12.17

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