Ingredient Analysis

Effects and aster water extract on proliferation and invasion of human lung cancer A549 cells, and tumorigenesis ability of nude mice*

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  • 1. Changsha Civil Affairs Vocational and Technical College, Changsha 410004, China;
    2. Department of Thoracic Surgery, Nanhua Hospital Affiliated to Nanhua University, Hengyang 421002, China;
    3. Department of Basic Nursing, School of Nursing, Nanhua University, Hengyang 421001, China;
    4. Department of Critical Care Medicine, Nanhua Hospital Affiliated to Nanhua University, Hengyang 421002, China

Received date: 2021-02-10

  Online published: 2024-06-26

Abstract

Objective: To explore the effects and mechanisms of aster water extract (AWE) on the proliferation and invasion of human lung cancer A549 cells, and the tumorigenesis capability of nude mice. Methods: MRC-5 and A549 cells were cultured in vitro and incubated with different concentrations of AWE (0, 0.6, 1.25, 2.5, 5, 10, 20, 40, 80, 160, 320 g·L-1). In the MTT assay, A549 cells were divided into AWE 0 g·L-1 group, AWE 10 g·L-1 group, AWE 20 g·L-1 group and AWE 40 g·L-1 group, and the cell proliferation rate in each group was detected by CCK-8. The number of invasion cells was detected using Transwell method. A549 cells were treated with Wnt/β-catenin signaling pathway inhibitor XAV939. The expression of β-catenin, vascular endothelial growth factor (VEGF) and Ki-67 proteins in each group was detected by Western blot. About 0.2 mL of A549 cells suspension (5×106 cell·mL-1) was subcutaneously implanted. After the intervention of AWE (5 g·kg-1·d-1 intragastric administration, 4 weeks), transplanted tumor tissues was weighed. The expression of VEGF and Ki-67 was detected by immunohistochemistry. Results: There was no significant inhibitory effect of AWE on MRC-5 cells (P>0.05), the inhibitory effect on A549 cells showed concentration-dependence (IC50=24.81 g·L-1). With the increase of AWE concentration, when AWE concentration was not lower than 20 g·L-1, survival rate of cells was decreased (P<0.05). The proliferation activity of A549 cells and number of invasion cells in AWE 20 g·L-1 group and AWE 40 g·L-1 group were lower than those in AWE 0 g·L-1 group (P<0.05). The expressions of Wnt-1, β-catenin, Ki67 and VEGF proteins in A549 cells of AWE 40 g·L-1 group and XAV939 group were lower than those in control group (P<0.05), which were lower in AWE 40 g·L-1+XAV939 group than AWE 40 g·L-1 group and XAV939 group (P<0.05). The results of tumorigenicity assay in nude mouse showed that the mass of transplanted tumors in AWE group was lower than that in control group (P<0.05), and the expression of Ki67 and VEGF proteins in transplanted tumor tissue was lower than that in control group (P<0.05). Conclusion: AWE can inhibit the proliferation and invasion of lung cancer cells, and inhibit growth of transplanted tumors, which may be related to the inhibition the expression of Wnt/β-catenin signaling pathway.

Cite this article

YAO Ping-bo . Effects and aster water extract on proliferation and invasion of human lung cancer A549 cells, and tumorigenesis ability of nude mice*[J]. Chinese Journal of Pharmaceutical Analysis, 2022 , 42(3) : 380 -386 . DOI: 10.16155/j.0254-1793.2022.03.03

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